RGD Reference Report - Acute effects of insulin on the activity of mitochondrial GPAT1 in primary adipocytes. - Rat Genome Database

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Acute effects of insulin on the activity of mitochondrial GPAT1 in primary adipocytes.

Authors: Bronnikov, GE  Aboulaich, N  Vener, AV  Stralfors, P 
Citation: Bronnikov GE, etal., Biochem Biophys Res Commun. 2008 Feb 29;367(1):201-7. Epub 2007 Dec 31.
RGD ID: 2313661
Pubmed: PMID:18167308   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2007.12.127   (Journal Full-text)

The mitochondrial enzyme 1-acyl-sn-glycerol-3-phosphate acyltransferase (mtGPAT1) catalyzes a rate-limiting step in triacylglycerol and glycerophospholipid biosynthesis, which can be modulated by protein kinases in cell free analyses. We report that treatment of primary rat adipocytes with insulin acutely affects the activity of mtGPAT1 by increasing V(MAX) and K(M) for the substrates glycerol-3-phosphate and palmitoyl-CoA. Proteolytic cleavage of isolated mitochondrial membranes and mass spectrometric peptide sequencing identify in vivo phosphorylation of serine 632 and serine 639 in mtGPAT1. These phosphorylation sites correspond to casein kinase-2 consensus sequences and are highly conserved in chordate animal, but not fly, fungal or plant, mtGPAT1.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GpamRatcellular response to insulin stimulus  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GpamRatmitochondrial membrane  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gpam  (glycerol-3-phosphate acyltransferase, mitochondrial)


Additional Information