RGD Reference Report - Sequential changes in the expression of genes involved in lipid metabolism in adipose tissue and liver in response to fasting. - Rat Genome Database

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Sequential changes in the expression of genes involved in lipid metabolism in adipose tissue and liver in response to fasting.

Authors: Palou, M  Priego, T  Sanchez, J  Villegas, E  Rodriguez, AM  Palou, A  Pico, C 
Citation: Palou M, etal., Pflugers Arch. 2008 Aug;456(5):825-36. Epub 2008 May 21.
RGD ID: 2313653
Pubmed: PMID:18493788   (View Abstract at PubMed)
DOI: DOI:10.1007/s00424-008-0461-1   (Journal Full-text)

The aim of this study was to provide a sequential analysis of the expression patterns of key genes involved in lipid metabolism in white adipose tissue (WAT) and liver and their relationship with blood parameters in response to fasting. Adult male rats were studied under different feeding conditions: feeding state, after 4, 8, or 24 h fasting, and after 3 h refeeding after 8 h fasting. Blood parameters and the expression of genes involved in lipogenesis and lipolysis in WAT and liver were analyzed. mRNA levels of genes involved in lipogenesis in liver (SREBP1c, FAS, and GPAT) had already decreased after 4 h fasting, as well as those of PPARgamma in WAT, whereas the decrease in SREBP1c, FAS, GPAT, and GLUT4 mRNA levels in WAT was observed after 8 h. Concerning lipolytic and fatty-acid-oxidation-related genes, liver PPARalpha, FGF21, CPT1, and PDK4 mRNA levels increased after 8 h fasting and those of ACOX1 after 24 h, and in WAT, ATGL, and CPT1 mRNA levels were greater after 24 h. Three hours refeeding increased the expression levels of PPARgamma in WAT, SREBP1c in both liver and WAT, and GPAT in liver, and decreased the expression levels of PPARalpha, CPT1, and PDK4 in liver. These results give new insight into the different adaptive time course response to fasting in the expression of genes involved in lipid metabolism, thus pointing out the very rapid response of lipogenic genes, particularly in liver, and the later response of lipolytic genes, particularly in WAT.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GpamRatresponse to nutrient levels  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gpam  (glycerol-3-phosphate acyltransferase, mitochondrial)


Additional Information