RGD Reference Report - Indomethacin enhances shuttling of aquaporin-2 despite decreased abundance in rat kidney. - Rat Genome Database

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Indomethacin enhances shuttling of aquaporin-2 despite decreased abundance in rat kidney.

Authors: Kim, SW  Kim, JW  Choi, KC  Ma, SK  Oh, Y  Jung, JY  Kim, J  Lee, J 
Citation: Kim SW, etal., J Am Soc Nephrol. 2004 Dec;15(12):2998-3005.
RGD ID: 2313211
Pubmed: PMID:15579502   (View Abstract at PubMed)
DOI: DOI:10.1097/01.ASN.0000145877.28811.82   (Journal Full-text)

The effect of nonsteroidal antiinflammatory drugs on the regulation of aquaporin-2 (AQP2) water channels in the kidney was determined. Male Sprague-Dawley rats were injected with indomethacin (5 mg/kg twice a day intraperitoneally) for 2 d. The control group was injected with vehicle. The expression of AQP2 proteins was determined in the kidney by immunoblotting and immunohistochemistry. The expression of G(salpha) and type VI adenylyl cyclase was determined by immunoblotting. The activity of adenylyl cyclase complexes was determined by stimulated accumulation of cAMP. Immunoblotting revealed that indomethacin markedly decreased the expression of AQP2. Accordingly, however, the ratio of AQP2 expression in the membrane fraction versus that in the cytoplasmic fraction was increased. The urinary excretion of AQP2 proteins also increased. Immunohistochemistry demonstrated almost exclusive apical labeling of AQP2 with scanty cytoplasmic localization along the collecting duct. The expression of G(salpha) and adenylyl cyclase VI proteins was decreased. The generation of cAMP provoked by arginine vasopressin, sodium fluoride, or forskolin was blunted. These results suggest that indomethacin increases the shuttling of AQP2 while it decreases its abundance in the collecting duct.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GnasRatadenylate cyclase-activating G protein-coupled receptor signaling pathway  IDA  RGD 
Adcy6RatcAMP biosynthetic process  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp2Ratapical plasma membrane  IDA  RGD 
Aqp2Ratbasolateral plasma membrane  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Adcy6Ratadenylate cyclase activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ADCY6HumanG protein mediated signaling pathway via Galphas family  ISOAdcy6 (Rattus norvegicus) RGD 
Adcy6MouseG protein mediated signaling pathway via Galphas family  ISOAdcy6 (Rattus norvegicus) RGD 
Adcy6RatG protein mediated signaling pathway via Galphas family  IDA  RGD 
GNASHumanG protein mediated signaling pathway via Galphas family  ISOGnas (Rattus norvegicus) RGD 
GnasRatG protein mediated signaling pathway via Galphas family  IDA  RGD 
GnasMouseG protein mediated signaling pathway via Galphas family  ISOGnas (Rattus norvegicus) RGD 
ADCY6Humanprotein kinase A (PKA) signaling pathway   ISOAdcy6 (Rattus norvegicus) RGD 
Adcy6Mouseprotein kinase A (PKA) signaling pathway   ISOAdcy6 (Rattus norvegicus) RGD 
Adcy6Ratprotein kinase A (PKA) signaling pathway   IDA  RGD 
ADCY6Humanvasopressin signaling pathway   ISOAdcy6 (Rattus norvegicus) RGD 
AQP2Humanvasopressin signaling pathway   ISOAqp2 (Rattus norvegicus) RGD 
Adcy6Mousevasopressin signaling pathway   ISOAdcy6 (Rattus norvegicus) RGD 
Adcy6Ratvasopressin signaling pathway   IDA  RGD 
Aqp2Mousevasopressin signaling pathway   ISOAqp2 (Rattus norvegicus) RGD 
Aqp2Ratvasopressin signaling pathway   IDA  RGD 
GNASHumanvasopressin signaling pathway   ISOGnas (Rattus norvegicus) RGD 
GnasRatvasopressin signaling pathway   IDA  RGD 
GnasMousevasopressin signaling pathway   ISOGnas (Rattus norvegicus) RGD 
ADCY6Humanvasopressin signaling pathway via receptor type 2   ISOAdcy6 (Rattus norvegicus) RGD 
AQP2Humanvasopressin signaling pathway via receptor type 2   ISOAqp2 (Rattus norvegicus) RGD 
Adcy6Mousevasopressin signaling pathway via receptor type 2   ISOAdcy6 (Rattus norvegicus) RGD 
Adcy6Ratvasopressin signaling pathway via receptor type 2   IDA  RGD 
Aqp2Mousevasopressin signaling pathway via receptor type 2   ISOAqp2 (Rattus norvegicus) RGD 
Aqp2Ratvasopressin signaling pathway via receptor type 2   IDA  RGD 
GNASHumanvasopressin signaling pathway via receptor type 2   ISOGnas (Rattus norvegicus) RGD 
GnasRatvasopressin signaling pathway via receptor type 2   IDA  RGD 
GnasMousevasopressin signaling pathway via receptor type 2   ISOGnas (Rattus norvegicus) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Adcy6  (adenylate cyclase 6)
Aqp2  (aquaporin 2)
Gnas  (GNAS complex locus)

Genes (Mus musculus)
Adcy6  (adenylate cyclase 6)
Aqp2  (aquaporin 2)
Gnas  (GNAS complex locus)

Genes (Homo sapiens)
ADCY6  (adenylate cyclase 6)
AQP2  (aquaporin 2)
GNAS  (GNAS complex locus)


Additional Information