RGD Reference Report - Manganese ion dependent adenylate cyclase activity in rat testes: purification and properties. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Manganese ion dependent adenylate cyclase activity in rat testes: purification and properties.

Authors: Kornblihtt, AR  Flawia, MM  Torres, HN 
Citation: Kornblihtt AR, etal., Biochemistry. 1981 Mar 3;20(5):1262-7.
RGD ID: 2313177
Pubmed: (View Article at PubMed) PMID:7225326

Testicular, soluble adenylate cyclase has been purified by anion-exchange chromatography, gel filtration, and isoelectric focusing. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peak fractions from the latter purification step showed only one polypeptide band with an apparent molecular weight of about 69 000. The following hydrodynamic and molecular parameters have been established for this enzyme: sedimentation constant, 4.3; Stokes radius, 3.95 nm; partial specific volume, 0.74 mL.g(-1); molecular weight, 74 000; fractional ratio, 1.4.

Annotation

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Adcy10  (adenylate cyclase 10)


Additional Information