RGD Reference Report - Fatty acid transport by vectorial acylation in mammals: roles played by different isoforms of rat long-chain acyl-CoA synthetases. - Rat Genome Database

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Fatty acid transport by vectorial acylation in mammals: roles played by different isoforms of rat long-chain acyl-CoA synthetases.

Authors: Tong, F  Black, PN  Coleman, RA  DiRusso, CC 
Citation: Tong F, etal., Arch Biochem Biophys. 2006 Mar 1;447(1):46-52. Epub 2006 Jan 23.
RGD ID: 2312800
Pubmed: (View Article at PubMed) PMID:16466685
DOI: Full-text: DOI:10.1016/j.abb.2006.01.005

Mammals express multiple isoforms of acyl-CoA synthetase (ACSL1 and ACSL3-6) in various tissues. These enzymes are essential for fatty acid metabolism providing activated intermediates for complex lipid synthesis, protein modification, and beta-oxidation. Yeast in contrast express four major ACSLs, which have well-defined functions. Two, Faa1p and Faa4p, are specifically required for fatty acid transport by vectorial acylation. Four ACSLs from the rat were expressed in a yeast faa1delta faa4delta strain and their roles in fatty acid transport and trafficking characterized. All four restored ACS activity yet varied in substrate preference. ACSL1, 4, and 6 were able to rescue fatty acid transport activity and triglyceride synthesis. ACSL5, however, was unable to facilitate fatty acid transport despite conferring robust oleoyl-CoA synthetase activity. This is the first study evaluating the role of the mammalian ACSLs in fatty acid transport and supports a role for ACSL1, 4, and 6 in transport by vectorial acylation.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Acsl1  (acyl-CoA synthetase long-chain family member 1)
Acsl4  (acyl-CoA synthetase long-chain family member 4)
Acsl5  (acyl-CoA synthetase long-chain family member 5)
Acsl6  (acyl-CoA synthetase long-chain family member 6)


Additional Information