RGD Reference Report - Angiotensin II regulates V2 receptor and pAQP2 during ureteral obstruction. - Rat Genome Database

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Angiotensin II regulates V2 receptor and pAQP2 during ureteral obstruction.

Authors: Jensen, AM  Bae, EH  Fenton, RA  Norregaard, R  Nielsen, S  Kim, SW  Frokiaer, J 
Citation: Jensen AM, etal., Am J Physiol Renal Physiol. 2009 Jan;296(1):F127-34. Epub 2008 Oct 29.
RGD ID: 2312654
Pubmed: (View Article at PubMed) PMID:18971210
DOI: Full-text: DOI:10.1152/ajprenal.90479.2008

Release of bilateral ureteral obstruction (BUO) is associated with nephrogenic diabetes insipidus (NDI) and a reduced abundance of the vasopressin-regulated aquaporins. To evaluate the role of the vasopressin type 2 receptor (V2R), we determined V2R abundance in kidneys from rats subjected to 24-h BUO or 24-h unilateral ureteral obstruction (UUO) followed by 48-h release. Because angiotensin II type 1 (AT1) receptor blockade attenuates postobstructive polyuria and aquaporin-2 (AQP2) downregulation, we examined the effect of AT1 receptor blockade on AQP2 phosphorylated at serine 256 (pS256-AQP2) and V2 receptor complex abundance in kidney inner medulla (IM). Furthermore, cAMP generation in sodium fluoride- and forskolin-stimulated inner medullary membrane fractions was studied after release of BUO. V2R was significantly reduced to 12% of sham levels in IM and to 52% of sham levels in cortex and outer stripe of outer medulla (OSOM) from BUO rats. In UUO rats, V2R abundance in the obstructed kidney IM decreased to 35% of sham levels, whereas it was comparable to sham levels in the nonobstructed kidney IM. No significant change was observed in cortex and OSOM. AT1 receptor blockade attenuated V2R, pS256-AQP2, and G(s)alpha protein downregulation in IM and partially reversed the obstruction-induced inhibition of sodium fluoride- and forskolin-stimulated cAMP generation in inner medullary membrane fractions from BUO rats. In conclusion, V2R downregulation plays a pivotal role in development of NDI after release of BUO. In addition, we have shown that angiotensin II regulates the V2 receptor complex and pS256-AQP2 in postobstructive kidney IM, probably by stimulating cAMP generation.


Disease Annotations    

Gene Ontology Annotations    

Biological Process

Cellular Component

Molecular Pathway Annotations    
Objects Annotated

Genes (Rattus norvegicus)
Adcy6  (adenylate cyclase 6)
Aqp2  (aquaporin 2)
Avpr2  (arginine vasopressin receptor 2)
Gnas  (GNAS complex locus)

Genes (Mus musculus)
Adcy6  (adenylate cyclase 6)
Aqp2  (aquaporin 2)
Avpr2  (arginine vasopressin receptor 2)
Gnas  (GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus)

Genes (Homo sapiens)
ADCY6  (adenylate cyclase 6)
AQP2  (aquaporin 2)
AVPR2  (arginine vasopressin receptor 2)
GNAS  (GNAS complex locus)

Additional Information