RGD Reference Report - Cellular and subcellular localization of PDE10A, a striatum-enriched phosphodiesterase. - Rat Genome Database

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Cellular and subcellular localization of PDE10A, a striatum-enriched phosphodiesterase.

Authors: Xie, Z  Adamowicz, WO  Eldred, WD  Jakowski, AB  Kleiman, RJ  Morton, DG  Stephenson, DT  Strick, CA  Williams, RD  Menniti, FS 
Citation: Xie Z, etal., Neuroscience. 2006 May 12;139(2):597-607. Epub 2006 Feb 17.
RGD ID: 2312515
Pubmed: PMID:16483723   (View Abstract at PubMed)
PMCID: PMC1464838   (View Article at PubMed Central)
DOI: DOI:10.1016/j.neuroscience.2005.12.042   (Journal Full-text)

PDE10A is a recently identified phosphodiesterase that is highly expressed by the GABAergic medium spiny projection neurons of the mammalian striatum. Inhibition of PDE10A results in striatal activation and behavioral suppression, suggesting that PDE10A inhibitors represent a novel class of antipsychotic agents. In the present studies we further elucidate the localization of this enzyme in striatum of rat and cynomolgus monkey. We find by confocal microscopy that PDE10A-like immunoreactivity is excluded from each class of striatal interneuron. Thus, the enzyme is restricted to the medium spiny neurons. Subcellular fractionation indicates that PDE10A is primarily membrane bound. The protein is present in the synaptosomal fraction but is separated from the postsynaptic density upon solubilization with 0.4% Triton X-100. Immuno-electron microscopy of striatum confirms that PDE10A is most often associated with membranes in dendrites and spines. Immuno-gold particles are observed on the edge of the postsynaptic density but not within this structure. Our studies indicate that PDE10A is associated with post-synaptic membranes of the medium spiny neurons, suggesting that the specialized compartmentation of PDE10A enables the regulation of intracellular signaling from glutamatergic and dopaminergic inputs to these neurons.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pde10aRatextrinsic component of postsynaptic density membrane is_active_inIDA PMID:16483723SynGO 
Pde10aRatextrinsic component of postsynaptic density membrane is_active_inIEP PMID:16483723SynGO 
Pde10aRatglutamatergic synapse is_active_inIDA PMID:16483723SynGO 
Pde10aRatglutamatergic synapse is_active_inIEP PMID:16483723SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Pde10a  (phosphodiesterase 10A)


Additional Information