RGD Reference Report - Novel identification of peripheral dopaminergic D2 receptor in male germ cells. - Rat Genome Database

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Novel identification of peripheral dopaminergic D2 receptor in male germ cells.

Authors: Otth, C  Torres, M  Ramirez, A  Fernandez, JC  Castro, M  Rauch, MC  Brito, M  Yanez, AJ  Rodriguez-Gil, JE  Slebe, JC  Concha, II 
Citation: Otth C, etal., J Cell Biochem. 2007 Jan 1;100(1):141-50.
RGD ID: 2311573
Pubmed: PMID:16924680   (View Abstract at PubMed)
DOI: DOI:10.1002/jcb.21037   (Journal Full-text)

Dopamine is a recognized modulator in the central nervous system (CNS) and peripheral organ functions. The presence of peripheral dopamine receptors outside the CNS has suggested an intriguing interaction between the nervous system and other functional systems, such as the reproductive system. In the present study we analyzed the expression of D2R receptors in rat testis, rat spermatogenic cells and spermatozoa, in different mammals. The RT-PCR analysis of rat testis mRNA showed specific bands corresponding to the two dopamine receptor D2R (L and S) isoforms previously described in the brain. Using Western blot analysis, we confirmed that the protein is present in rat testis, isolated spermatogenic cells and also in spermatozoa of a range of different mammals, such as rat, mouse, bull, and human. The immunohistochemistry analysis of rat adult testis showed that the receptor was expressed in all germ cells (pre- and post-meiotic phase) of the tubule with staining predominant in spermatogonia. Confocal analysis by indirect immunofluorescence revealed that in non-capacitated spermatozoa of rat, mouse, bull, and human, D2R is mainly localized in the flagellum, and is also observed in the acrosomal region of the sperm head (except in human spermatozoa). Our findings demonstrate that the two D2 receptor isoforms are expressed in rat testis and that the receptor protein is present in different mammalian spermatozoa. The presence of D2R receptors in male germ cells implies new and unsuspected roles for dopamine signaling in testicular and sperm physiology.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
acrosomal vesicle  IDA 2311573acrosomal region of spermatozoaRGD 
sperm flagellum  IDA 2311573 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Drd2  (dopamine receptor D2)


Additional Information