RGD Reference Report - Identification of glucose-dependant insulin secretion targets in pancreatic beta cells by combining defined-mechanism compound library screening and siRNA gene silencing. - Rat Genome Database

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Identification of glucose-dependant insulin secretion targets in pancreatic beta cells by combining defined-mechanism compound library screening and siRNA gene silencing.

Authors: Wu, W  Shang, J  Feng, Y  Thompson, CM  Horwitz, S  Thompson, JR  MacIntyre, ED  Thornberry, NA  Chapman, K  Zhou, YP  Howard, AD  Li, J 
Citation: Wu W, etal., J Biomol Screen. 2008 Feb;13(2):128-34. Epub 2008 Jan 23.
RGD ID: 2311570
Pubmed: PMID:18216393   (View Abstract at PubMed)
DOI: DOI:10.1177/1087057107313763   (Journal Full-text)

Identification and validation of novel drug targets continues to be a major bottleneck in drug development, particularly for polygenic complex diseases such as type 2 diabetes. Here, the authors describe an approach that allows researchers to rapidly identify and validate potential drug targets by combining chemical tools and RNA interference technology. As a proof-of-concept study, the known mechanism Sigma LOPAC library was used to screen for glucose-dependent insulin secretion (GDIS) in INS-1 832/13 cells. In addition to several mechanisms that are known to regulate GDIS (such as cyclic adenosine monophosphate-specific phosphodiesterases, adrenoceptors, and Ca(2+) channels), the authors find that several of the dopamine receptor (DRD) antagonists significantly enhance GDIS, whereas DRD agonists profoundly inhibit GDIS. Subsequent siRNA studies in the same cell line indicate that knockdown of DRD2 enhanced GDIS. Furthermore, selective DRD2 antagonists and agonists also enhance or suppress, respectively, GDIS in isolated rat islets. The data support that the approach described here offers a rapid and effective way for target identification and validation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of insulin secretion  IMP 2311570 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Drd2  (dopamine receptor D2)


Additional Information