RGD Reference Report - Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells. - Rat Genome Database

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Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells.

Authors: Fukuda, M  Kowalchyk, JA  Zhang, X  Martin, TF  Mikoshiba, K 
Citation: Fukuda M, etal., J Biol Chem. 2002 Feb 15;277(7):4601-4. Epub 2001 Dec 21.
RGD ID: 2311146
Pubmed: PMID:11751925   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.C100588200   (Journal Full-text)

Synaptotagmin (Syt) I-deficient phaeochromocytoma (PC12) cell lines show normal Ca(2+)-dependent norepinephrine (NE) release (Shoji-Kasai, Y., Yoshida, A., Sato, K., Hoshino, T., Ogura, A., Kondo, S., Fujimoto, Y., Kuwahara, R., Kato, R., and Takahashi, M. (1992) Science 256, 1821-1823). To identify an alternative Ca(2+) sensor, we searched for other Syt isoforms in Syt I-deficient PC12 cells and identified Syt IX, an isoform closely related to Syt I, as an abundantly expressed dense-core vesicle protein. Here we show that Syt IX is required for the Ca(2+)-dependent release of NE from PC12 cells. Antibodies directed against the C2A domain of either Syt IX or Syt I inhibited Ca(2+)-dependent NE release in permeable PC12 cells indicating that both Syt proteins function in dense-core vesicle exocytosis. Our results support the idea that Syt family proteins that co-reside on secretory vesicles may function cooperatively and redundantly as potential Ca(2+) sensors for exocytosis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Syt1Ratregulation of calcium ion-dependent exocytosis  IMP  RGD 
Syt9Ratregulation of calcium ion-dependent exocytosis  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Syt1Ratdense core granule  IDA  RGD 
Syt9Ratdense core granule  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Objects Annotated

Genes (Rattus norvegicus)
Syt1  (synaptotagmin 1)
Syt9  (synaptotagmin 9)

Genes (Mus musculus)
Syt1  (synaptotagmin I)
Syt9  (synaptotagmin IX)

Genes (Homo sapiens)
SYT1  (synaptotagmin 1)
SYT9  (synaptotagmin 9)


Additional Information