RGD Reference Report - Altered expression of epithelial sodium channel in rats with bilateral or unilateral ureteral obstruction. - Rat Genome Database

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Altered expression of epithelial sodium channel in rats with bilateral or unilateral ureteral obstruction.

Authors: Li, C  Wang, W  Norregaard, R  Knepper, MA  Nielsen, S  Frokiaer, J 
Citation: Li C, etal., Am J Physiol Renal Physiol. 2007 Jul;293(1):F333-41. Epub 2007 May 2.
RGD ID: 2308940
Pubmed: PMID:17475897   (View Abstract at PubMed)
DOI: DOI:10.1152/ajprenal.00372.2006   (Journal Full-text)

The roles of epithelial sodium channel (ENaC) subunits (alpha, beta, and gamma) in the impaired renal reabsorption of sodium and water were examined in rat models with bilateral (BUO) or unilateral ureteral obstruction (UUO) for 24 h or with BUO followed by release of obstruction and 3 days of observation (BUO-3dR). In BUO rats, plasma osmolality was increased dramatically, whereas plasma sodium concentration was decreased. Immunoblotting revealed a significantly decreased expression of alpha-ENaC (57 +/- 7%), beta-ENaC (19 +/- 5%), and gamma-ENaC (51 +/- 10%) as well as 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2) in the cortex and outer medulla (C+OM) compared with sham-operated controls. This was confirmed by immunohistochemistry. BUO-3dR was associated with polyuria and impaired renal sodium handling. The protein abundance and the apical labeling of alpha-ENaC were significantly increased, whereas beta- and gamma-ENaC as well as 11beta-HSD2 expression remained decreased. In UUO rats, expression of alpha- and beta-ENaC and 11beta-HSD2 decreased in the C+OM in the obstructed kidney. In contrast, the abundance and the apical labeling of alpha-ENaC in the nonobstructed kidneys were markedly increased, suggesting compensatory upregulation in this kidney. In conclusion, alpha-, beta-, and gamma-ENaC expression levels are downregulated in the obstructed kidney. The expression and apical labeling of alpha-ENaC were increased in BUO-3dR rats and in the nonobstructed kidneys from UUO rats. These results suggest that altered expression of alpha-, beta-, and gamma-ENaC may contribute to impaired renal sodium and water handling in response to ureteral obstruction.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HSD11B2Humanureteral obstruction  ISOHsd11b2 (Rattus norvegicus)protein:decreased expression:kidneyRGD 
Hsd11b2Ratureteral obstruction  IEP protein:decreased expression:kidneyRGD 
Hsd11b2Mouseureteral obstruction  ISOHsd11b2 (Rattus norvegicus)protein:decreased expression:kidneyRGD 

Objects Annotated

Genes (Rattus norvegicus)
Hsd11b2  (hydroxysteroid 11-beta dehydrogenase 2)

Genes (Mus musculus)
Hsd11b2  (hydroxysteroid 11-beta dehydrogenase 2)

Genes (Homo sapiens)
HSD11B2  (hydroxysteroid 11-beta dehydrogenase 2)


Additional Information