RGD Reference Report - Effects of prostaglandin E2 and indomethacin on 25-hydroxyvitamin D3-1 alpha-hydroxylase activity in isolated kidney cells of normal and streptozotocin-induced diabetic rats. - Rat Genome Database

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Effects of prostaglandin E2 and indomethacin on 25-hydroxyvitamin D3-1 alpha-hydroxylase activity in isolated kidney cells of normal and streptozotocin-induced diabetic rats.

Authors: Kurose, H  Sonn, YM  Jafari, A  Birge, SJ  Avioli, LV 
Citation: Kurose H, etal., Calcif Tissue Int. 1985 Dec;37(6):625-9.
RGD ID: 2307314
Pubmed: PMID:3937586   (View Abstract at PubMed)

The effects of prostaglandin E2 (PGE2) (1 microM) and indomethacin (IN) (20 microM) on 1,25 dihydroxyvitamin D3 production were studied in renal cell suspensions isolated from control, streptozotocin-induced diabetic, and insulin-treated diabetic rats. Renal cortex cells were isolated by enzymatic digestion, and preincubated for 30 min at 37 degrees C with the appropriate additive(s) followed by a 1 h incubation with 8 nM 25-hydroxyvitamin D3 in serum-free medium. Radioactivity incorporated into that fraction of the cell suspension extract co-eluting with synthetic 1,25-dihydroxyvitamin D3 on high pressure liquid chromatography was determined. All animals were raised for 5 weeks on a vitamin D-deficient diet. Isolated kidney cells from vitamin D-deficient rats showed dose-dependent response of 1,25 dihydroxyvitamin D3 production to PGE2. Cells from control animals demonstrated a stimulatory effect of PGE2 (P less than 0.05) and a suppressive effect of IN (P less than 0.01) on 1,25 dihydroxyvitamin D3 production. In contrast, cells from diabetic rat kidneys failed to respond to these agents, alterations which were reversed by insulin treatment. The accumulated data suggest that depressed synthesis of 1,25 dihydroxyvitamin D3 previously observed in the experimental diabetic rat is due, at least in part, to an impaired production and response to PGE2-like prostaglandins.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to prostaglandin E  IDA 2307314 RGD 
response to xenobiotic stimulus  IDA 2307314indomethacinRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp27b1  (cytochrome P450, family 27, subfamily b, polypeptide 1)


Additional Information