RGD Reference Report - Impact of monocyte chemoattractant protein-1 deficiency on cerebral aneurysm formation. - Rat Genome Database
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Impact of monocyte chemoattractant protein-1 deficiency on cerebral aneurysm formation.

Authors: Aoki, T  Kataoka, H  Ishibashi, R  Nozaki, K  Egashira, K  Hashimoto, N 
Citation: Aoki T, etal., Stroke. 2009 Mar;40(3):942-51. Epub 2009 Jan 22.
RGD ID: 2307000
Pubmed: (View Article at PubMed) PMID:19164781
DOI: Full-text: DOI:10.1161/STROKEAHA.108.532556

BACKGROUND AND PURPOSE: Recent studies have suggested that chronic inflammation actively participates in cerebral aneurysm (CA) formation. Macrophages accumulate in CA walls and express proinflammatory genes promoting CA progression, but the molecular mechanisms of monocyte/macrophage recruitment into CA walls remain to be elucidated. METHODS: Monocyte chemoattractant protein-1 (MCP-1) expression in experimentally induced CAs was assessed by immunohistochemistry and Western blotting. The role of MCP-1 in CA formation was examined by MCP-1(-/-) mice and a plasmid DNA encoding a dominant negative mutant of MCP-1 (7ND). MCP-1 expression in human CAs was examined by immunohistochemistry. RESULTS: MCP-1 expression was upregulated in aneurysmal walls at the early stage of CA formation. MCP-1(-/-) mice exhibited a significant decrease of CA formation and macrophage accumulation with decreased expression of matrix metalloproteinase-2, -9, and inducible nitric oxide synthase. Immunohistochemistry for the DNA binding form of nuclear factor-kappa B showed nuclear factor-kappa B activation in MCP-1-expressing cells. Blockade of MCP-1 activity by 7ND resulted in the inhibition of CA progression in rats. In human CAs, MCP-1 was also expressed in CA walls. CONCLUSIONS: These data suggest that MCP-1 plays a crucial role in CA formation as a major chemoattractant for monocyte/macrophage. MCP-1 expression in CA walls is induced through nuclear factor-kappa B activation. MCP-1 may be a novel therapeutic target of medical treatment preventing CA progression.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Ccl2  (C-C motif chemokine ligand 2)

Genes (Mus musculus)
Ccl2  (chemokine (C-C motif) ligand 2)

Genes (Homo sapiens)
CCL2  (C-C motif chemokine ligand 2)

Objects referenced in this article
0 CCL13 C-C motif chemokine ligand 13 All species

Additional Information