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Selenium requirements are higher for glutathione peroxidase-1 mRNA than gpx1 activity in rat testis.

Authors: Schriever, SC  Barnes, KM  Evenson, JK  Raines, AM  Sunde, RA 
Citation: Schriever SC, etal., Exp Biol Med (Maywood). 2009 May;234(5):513-21. Epub 2009 Feb 20.
Pubmed: (View Article at PubMed) PMID:19234057
DOI: Full-text: DOI:10.3181/0812-RM-369

Selenium (Se) plays a critical role in testis, sperm, and reproduction, and testis Se levels are remarkably maintained in Se deficiency. In most other tissues, Se levels decrease dramatically as do levels of most selenoproteins and levels of a subset of Se-regulated selenoprotein mRNAs. Because of the recent identification of key molecules in the targeted trafficking of Se to the testis, we examined the hierarchy of Se regulation in testis by determining the dietary Se regulation of the full testis selenoproteome in rats fed graded levels of Se (0 to 0.8 mug Se/g) as Na(2)SeO(3) for 28 d. Se status did not significantly affect testis weight or glutathione peroxidase 4 (Gpx4) activity (P > 0.05). qRT-PCR analysis of selenoprotein mRNA expression revealed that 21 of the 24 selenoprotein mRNAs and ApoER2 mRNA (the selenoprotein P [Sepp1] receptor) were also not regulated significantly by dietary Se status. In contrast, Gpx1 activity decreased to 28% of Se-adequate levels, and mRNA levels for Gpx1, Sepp1, and Sepw1 (selenoprotein W) decreased significantly in Se-deficient rats to 45, 46, and 55%, respectively, of Se-adequate plateau levels. Overlap of hyperbolic Gpx4 activity and Sepw1 mRNA response curves with testis Se concentration, all with minimum dietary Se requirements <0.016 mug Se/g, showed the priority for synthesis of Gpx4. Higher minimum dietary Se requirements of 0.04 mug Se/g for Gpx1 activity and Sepp1 mRNA, and the even higher minimum dietary Se requirement of 0.08 mug Se/g for Gpx1 mRNA, suggest that the hierarchy of these biomarkers reflects distinct, lower priority pools, cell types, and roles for Se within the testis.

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RGD Object Information
RGD ID: 2306614
Created: 2009-04-28
Species: All species
Last Modified: 2009-04-28
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.