RGD Reference Report - Periglomerular accumulation of dendritic cells in rat crescentic glomerulonephritis. - Rat Genome Database

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Periglomerular accumulation of dendritic cells in rat crescentic glomerulonephritis.

Authors: Fujinaka, H  Nameta, M  Kovalenko, P  Matsuki, A  Kato, N  Nishimoto, G  Yoshida, Y  Yaoita, E  Naito, M  Kihara, I  Tomizawa, S  Yamamoto, T 
Citation: Fujinaka H, etal., J Nephrol. 2007 May-Jun;20(3):357-63.
RGD ID: 2306579
Pubmed: PMID:17557270   (View Abstract at PubMed)

BACKGROUND: An increased number of major histocompatibility complex (MHC) class II-positive cells (OX-6+ cells) were observed in the glomerulus and periglomerular interstitium during the course of anti-glomerular basement membrane (anti-GBM) crescentic glomerulonephritis (GN) in WKY rats. This study aimed to demonstrate that periglomerular OX-6+ cells are dendritic cells (DCs) and to clarify their roles in the pathogenesis of this GN. METHODS: Kidney sections were stained with the OX-6 and the rat DC marker OX-62 by immunohistochemistry, and periglomerular OX-6+ cells were observed by immunoelectron microscopy. Renal mRNA expression for CXCL12 was examined by reverse transcriptase polymerase chain reaction (RT-PCR) and in situ hybridization, and that for IL-1beta was examined by in situ hybridization. RESULTS: Immunohistochemistry revealed that most periglomerular OX-6+ cells in this GN were ED-1-negative. OX-62+ cells were observed sparsely in normal kidney interstitium, and considerably more frequently in periglomerular interstitium in this GN. Immunoelectron microscopy confirmed the periglomerular OX-6+ED-1- cells had DC morphology. The increased expression of CXCL12 mRNA in the diseased glomerulus was shown by RT-PCR. By in situ hybridization, CXCL12 mRNA-expressing glomerular cells were the parietal and visceral epithelial cells, which were close to the site of periglomerular OX-6+ cell localization. The intense expression of IL-1beta mRNA by periglomerular cells was demonstrated by in situ hybridization. CONCLUSIONS: The periglomerular distribution of OX-6+ED-1- DCs was demonstrated in anti-GBM crescentic GN in WKY rats. These DCs might be accumulated in periglomerular interstitium by CXCL12, and play a role in the initiation and progression of this GN by producing IL-1beta.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
anti-basement membrane glomerulonephritis  ISOCxcl12 (Rattus norvegicus)2306579; 2306579mRNA:increased expression:glomerulusRGD 
anti-basement membrane glomerulonephritis  IEP 2306579mRNA:increased expression:glomerulusRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcl12  (C-X-C motif chemokine ligand 12)

Genes (Mus musculus)
Cxcl12  (C-X-C motif chemokine ligand 12)

Genes (Homo sapiens)
CXCL12  (C-X-C motif chemokine ligand 12)


Additional Information