RGD Reference Report - A novel glycogen-targeting subunit of protein phosphatase 1 that is regulated by insulin and shows differential tissue distribution in humans and rodents. - Rat Genome Database

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A novel glycogen-targeting subunit of protein phosphatase 1 that is regulated by insulin and shows differential tissue distribution in humans and rodents.

Authors: Munro, S  Ceulemans, H  Bollen, M  Diplexcito, J  Cohen, PT 
Citation: Munro S, etal., FEBS J. 2005 Mar;272(6):1478-89.
RGD ID: 2306166
Pubmed: (View Article at PubMed) PMID:15752363
DOI: Full-text: DOI:10.1111/j.1742-4658.2005.04585.x

Stimulation of glycogen-targeted protein phosphatase 1 (PP1) activity by insulin contributes to the dephosphorylation and activation of hepatic glycogen synthase (GS) leading to an increase in glycogen synthesis. The glycogen-targeting subunits of PP1, GL and R5/PTG, are downregulated in the livers of diabetic rodents and restored by insulin treatment. We show here that the mammalian gene PPP1R3E encodes a novel glycogen-targeting subunit of PP1 that is expressed in rodent liver. The phosphatase activity associated with R3E is slightly higher than that associated with R5/PTG and it is downregulated in streptozotocin-induced diabetes by 60-70% and restored by insulin treatment. Surprisingly, although mRNA for R3E is most highly expressed in rat liver and heart muscle, with only low levels in skeletal muscle, R3E mRNA is most abundant in human skeletal muscle and heart tissues with barely detectable levels in human liver. This species-specific difference in R3E mRNA expression has similarities to the high level of expression of GL mRNA in human but not rodent skeletal muscle. The observations imply that the mechanisms by which insulin regulates glycogen synthesis in liver and skeletal muscle are different in rodents and humans.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component

Molecular Function

Molecular Pathway Annotations    
Objects Annotated

Genes (Rattus norvegicus)
Ppp1r3b  (protein phosphatase 1, regulatory subunit 3B)
Ppp1r3c  (protein phosphatase 1, regulatory subunit 3C)
Ppp1r3e  (protein phosphatase 1, regulatory subunit 3E)

Genes (Mus musculus)
Ppp1r3e  (protein phosphatase 1, regulatory subunit 3E)

Genes (Homo sapiens)
PPP1R3E  (protein phosphatase 1 regulatory subunit 3E)


Additional Information