RGD Reference Report - Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography. - Rat Genome Database

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Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography.

Authors: Moorhead, G  MacKintosh, C  Morrice, N  Cohen, P 
Citation: Moorhead G, etal., FEBS Lett. 1995 Apr 3;362(2):101-5.
RGD ID: 2304267
Pubmed: (View Article at PubMed) PMID:7720853

The form of protein phosphatase-1 associated with hepatic glycogen (PP1G) was purified to near homogeneity from rat liver by affinity chromatography on microcystin-Sepharose and gel-filtration. The enzyme is a heterodimer consisting of the catalytic subunit of PP1 (the alpha and beta isoforms) complexed to a 33 kDa glycogen-binding (GL) subunit. The GL subunit binds phosphorylase a with high affinity, and is responsible for the enhanced dephosphorylation of glycogen synthase by PP1G and its allosteric inhibition by phosphorylase a.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component

Molecular Function

Molecular Pathway Annotations    
Objects Annotated

Genes (Rattus norvegicus)
Ppp1ca  (protein phosphatase 1 catalytic subunit alpha)
Ppp1cb  (protein phosphatase 1 catalytic subunit beta)
Ppp1r3b  (protein phosphatase 1, regulatory subunit 3B)

Genes (Mus musculus)
Ppp1ca  (protein phosphatase 1 catalytic subunit alpha)
Ppp1cb  (protein phosphatase 1 catalytic subunit beta)
Ppp1r3b  (protein phosphatase 1, regulatory subunit 3B)

Genes (Homo sapiens)
PPP1CA  (protein phosphatase 1 catalytic subunit alpha)
PPP1CB  (protein phosphatase 1 catalytic subunit beta)
PPP1R3B  (protein phosphatase 1 regulatory subunit 3B)


Additional Information