RGD Reference Report - Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography.

Authors: Moorhead, G  MacKintosh, C  Morrice, N  Cohen, P 
Citation: Moorhead G, etal., FEBS Lett. 1995 Apr 3;362(2):101-5.
RGD ID: 2304267
Pubmed: PMID:7720853   (View Abstract at PubMed)

The form of protein phosphatase-1 associated with hepatic glycogen (PP1G) was purified to near homogeneity from rat liver by affinity chromatography on microcystin-Sepharose and gel-filtration. The enzyme is a heterodimer consisting of the catalytic subunit of PP1 (the alpha and beta isoforms) complexed to a 33 kDa glycogen-binding (GL) subunit. The GL subunit binds phosphorylase a with high affinity, and is responsible for the enhanced dephosphorylation of glycogen synthase by PP1G and its allosteric inhibition by phosphorylase a.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glycogen granule  IDA 2304267; 2304267; 2304267 RGD 
protein phosphatase type 1 complex  IDA 2304267; 2304267; 2304267 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
enzyme binding  IDA 2304267 RGD 
phosphoprotein phosphatase activity  IDA 2304267; 2304267 RGD 
phosphoprotein phosphatase activity contributes_toIDA 2304267 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View
Objects Annotated

Genes (Rattus norvegicus)
Ppp1ca  (protein phosphatase 1 catalytic subunit alpha)
Ppp1cb  (protein phosphatase 1 catalytic subunit beta)
Ppp1r3b  (protein phosphatase 1, regulatory subunit 3B)

Genes (Mus musculus)
Ppp1ca  (protein phosphatase 1 catalytic subunit alpha)
Ppp1cb  (protein phosphatase 1 catalytic subunit beta)
Ppp1r3b  (protein phosphatase 1, regulatory subunit 3B)

Genes (Homo sapiens)
PPP1CA  (protein phosphatase 1 catalytic subunit alpha)
PPP1CB  (protein phosphatase 1 catalytic subunit beta)
PPP1R3B  (protein phosphatase 1 regulatory subunit 3B)


Additional Information