RGD Reference Report - Electron microscopical study of a cytosolic enzyme in unfixed cryostat sections: demonstration of glycogen phosphorylase activity in rat liver and heart tissue. - Rat Genome Database

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Electron microscopical study of a cytosolic enzyme in unfixed cryostat sections: demonstration of glycogen phosphorylase activity in rat liver and heart tissue.

Authors: Schellens, JP  Vreeling-Sindelarova, H  Van den Munckhof, RJ  Frederiks, WM 
Citation: Schellens JP, etal., Histochem J. 1995 Aug;27(8):609-14.
RGD ID: 2304119
Pubmed: PMID:8550381   (View Abstract at PubMed)

Glycogen phosphorylase activity has been demonstrated at the ultrastructural level in liver and heart tissue of fasted rats. Unfixed cryostat sections were incubated by mounting them on a semipermeable membrane stretched over a gelled incubation medium. The medium contained a high concentration of glucose 1-phosphate which enables indirect detection of glycogen phosphorylase activity on the basis of the synthesis of glycogen. Tissue fixation, dehydration and embedding for electron microscopical study were performed after the incubation had been completed. The ultrastructure of both liver and heart tissue was rather well preserved. Glycogen granules resulting from glycogen phosphorylase activity were found in the cytoplasmic matrix of both hepatocytes and cardiomyocytes; no relationship with membranous structures could be detected. It is concluded that the semipermeable membrane method is well suited for localizing cytosolic enzyme activities at the ultrastructural level without prior tissue fixation; this opens further perspectives for correlations between histochemical and biochemical data.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PyglRatglycogen metabolic process  IDA  RGD 
PygmRatglycogen metabolic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PyglRatglycogen phosphorylase activity  IDA  RGD 
PygmRatglycogen phosphorylase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pygl  (glycogen phosphorylase L)
Pygm  (glycogen phosphorylase, muscle associated)


Additional Information