RGD Reference Report - No limiting role for glycogenin in determining maximal attainable glycogen levels in rat skeletal muscle. - Rat Genome Database

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No limiting role for glycogenin in determining maximal attainable glycogen levels in rat skeletal muscle.

Authors: Hansen, BF  Derave, W  Jensen, P  Richter, EA 
Citation: Hansen BF, etal., Am J Physiol Endocrinol Metab. 2000 Mar;278(3):E398-404.
RGD ID: 2304070
Pubmed: PMID:10710493   (View Abstract at PubMed)

We examined whether the protein level and/or activity of glycogenin, the protein core upon which glycogen is synthesized, is limiting for maximal attainable glycogen levels in rat skeletal muscle. Glycogenin activity was 27.5 +/- 1.4, 34.7 +/- 1.7, and 39.7 +/- 1.3 mU/mg protein in white gastrocnemius, red gastrocnemius, and soleus muscles, respectively. A similar fiber type dependency of glycogenin protein levels was seen. Neither glycogenin protein level nor the activity of glycogenin correlated with previously determined maximal attainable glycogen levels, which were 69.3 +/- 5.8, 137.4 +/- 10.1, and 80.0 +/- 5.4 micromol/g wet wt in white gastrocnemius, red gastrocnemius, and soleus muscles, respectively. In additional experiments, rats were exercise trained by swimming, which resulted in a significant increase in the maximal attainable glycogen levels in soleus muscles ( approximately 25%). This increase in maximal glycogen levels was not accompanied by an increase in glycogenin protein level or activity. Furthermore, even in the presence of very high glycogen levels ( approximately 170 micromol/g wet wt), approximately 30% of the total glycogen pool continued to be present as unsaturated glycogen molecules (proglycogen). Therefore, it is concluded that glycogenin plays no limiting role for maximal attainable glycogen levels in rat skeletal muscle.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gyg1Ratglycogen biosynthetic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gyg1RatD-glucose binding  IDA UDP-glucoseRGD 
Gyg1Ratglycogenin glucosyltransferase activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GYG1Humanglycogen biosynthetic pathway   ISOGyg1 (Rattus norvegicus) RGD 
Gyg1Ratglycogen biosynthetic pathway   IDA  RGD 
Gyg1Mouseglycogen biosynthetic pathway   ISOGyg1 (Rattus norvegicus) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Gyg1  (glycogenin 1)

Genes (Mus musculus)
Gyg1  (glycogenin 1)

Genes (Homo sapiens)
GYG1  (glycogenin 1)


Additional Information