RGD Reference Report - Analysis of biological activities of recombinant rat interleukin-5. - Rat Genome Database

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Analysis of biological activities of recombinant rat interleukin-5.

Authors: Ishihara, K  Nittoh, T  Satoh, I  Ohuchi, K 
Citation: Ishihara K, etal., Int Arch Allergy Immunol. 1999;120 Suppl 1:11-4.
RGD ID: 2303750
Pubmed: (View Article at PubMed) PMID:10529595
DOI: Full-text: DOI:53585

We analyzed the biological activities of recombinant rat interleukin-5 (IL-5). Purified recombinant rat IL-5 promoted the proliferation of T88-M cells in a concentration-dependent manner, and its effect was inhibited by an anti-murine IL-5-neutralizing polyclonal antibody. When bone marrow cells from normal rats were incubated with recombinant rat IL-5 in medium containing methylcellulose, the colony formation by eosinophilic cells was induced. Furthermore, when rat peritoneal eosinophils were incubated with recombinant rat IL-5, the spontaneous decrease in eosinophil viability was inhibited in a time- and concentration-dependent manner. In addition, the recombinant rat IL-5-induced eosinophil survival was inhibited by an anti-murine IL-5-neutralizing polyclonal antibody. These findings suggest that rat IL-5 acts as B cell growth factor II, eosinophil differentiation factor, and eosinophil survival-enhancing factor.

Annotation

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Il5  (interleukin 5)


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