RGD Reference Report - Cloning and characterization of the rat 5-HT5B receptor. Evidence that the 5-HT5B receptor couples to a G protein in mammalian cell membranes. - Rat Genome Database

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Cloning and characterization of the rat 5-HT5B receptor. Evidence that the 5-HT5B receptor couples to a G protein in mammalian cell membranes.

Authors: Wisden, W  Parker, EM  Mahle, CD  Grisel, DA  Nowak, HP  Yocca, FD  Felder, CC  Seeburg, PH  Voigt, MM 
Citation: Wisden W, etal., FEBS Lett. 1993 Oct 25;333(1-2):25-31.
RGD ID: 2303647
Pubmed: PMID:8224165   (View Abstract at PubMed)

A gene encoding a novel G protein-coupled 5-hydroxytryptamine (5-HT) receptor, termed 5-HT5B, was cloned. The ligand binding profile of this receptor is distinct from that of other cloned 5-HT receptors. The 5-HT5B receptor couples to a G protein in COS1 cell membranes; however, activation of the 5-HT5B receptor does not appear to alter either cAMP accumulation or phosphoinositide turnover in a variety of fibroblast cell lines. In the rat brain, 5-HT5B gene expression occurs predominantly in the medial habenulae and hippocampal CA1 cells of the adult. Little expression is seen during embryonic development.

Gene-Chemical Interaction Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
5-carboxamidotryptamine affects bindingEXP 23036475-carboxamidotryptamine binds to 5-Htr5bRGD 
8-OH-DPAT multiple interactionsEXP 23036478-OH-DPAT binds to protein 5-Htr5b and inhibits the reaction[5-carboxamidotryptamine binds to 5-Htr5b]RGD 
dihydroergotamine multiple interactionsEXP 2303647dihydroergotamine binds to protein 5-Htr5b and inhibits the reaction[5-carboxamidotryptamine binds to 5-Htr5b]RGD 
lysergic acid diethylamide multiple interactionsEXP 2303647lysergic acid diethylamide binds to protein 5-Htr5b and inhibits the reaction[5-carboxamidotryptamine binds to 5-Htr5b]RGD 
methiothepin multiple interactionsEXP 2303647methiothepin binds to protein 5-Htr5b and inhibits the reaction[5-carboxamidotryptamine binds to 5-Htr5b]RGD 
methysergide multiple interactionsEXP 2303647methysergide binds to protein 5-Htr5b and inhibits the reaction[5-carboxamidotryptamine binds to 5-Htr5b]RGD 
trisodium guanosine 5'-[beta,gamma-imido]triphosphate multiple interactionsEXP 2303647trisodium guanosine 5'-[beta more ...RGD 
tryptamine alkaloid multiple interactionsEXP 23036475-hydroxytryptamine binds to protein 5-Htr5b and inhibits the reaction[5-carboxamidotryptamine binds to 5-Htr5b]RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
G protein-coupled receptor binding  IDA 2303647 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Htr5b  (5-hydroxytryptamine (serotonin) receptor 5B)


Additional Information