RGD Reference Report - Generation of rat eosinophils by recombinant rat interleukin-5 in vitro and in vivo. - Rat Genome Database

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Generation of rat eosinophils by recombinant rat interleukin-5 in vitro and in vivo.

Authors: Ishihara, K  Satoh, I  Mue, S  Ohuchi, K 
Citation: Ishihara K, etal., Biochim Biophys Acta. 2000 Apr 15;1501(1):25-32.
RGD ID: 2303509
Pubmed: (View Article at PubMed) PMID:10727846

The addition of recombinant rat interleukin-5 (IL-5), which was purified from the hemolymph of silkworm Bombyx mori larvae infected with IL-5-expressing recombinant virus, to cultures of rat bone marrow cells resulted in an increase in the number of Luxol-fast-blue staining eosinophils in a time- and concentration-dependent manner. After 6 days culture with 100 pM recombinant rat IL-5, more than 90% of the bone marrow cells were eosinophil. The contents of major basic protein (MBP) in the bone marrow cells determined by Western blot analysis using a polyclonal antibody to rat MBP were also increased by recombinant rat IL-5 (100 pM). Furthermore, intravenous injections of recombinant rat IL-5 twice a day for six consecutive days increased the population of eosinophils in peripheral blood cells and in bone marrow cells. These findings indicate that rat IL-5 induces terminal differentiation and proliferation of progenitor cells to mature eosinophils in rats.

Annotation

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Il5  (interleukin 5)


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