RGD Reference Report - Gene expression from the imprinted Dio3 locus is associated with cell proliferation of cultured brown adipocytes. - Rat Genome Database

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Gene expression from the imprinted Dio3 locus is associated with cell proliferation of cultured brown adipocytes.

Authors: Hernandez, A  Garcia, B  Obregon, MJ 
Citation: Hernandez A, etal., Endocrinology. 2007 Aug;148(8):3968-76. Epub 2007 May 17.
RGD ID: 2303418
Pubmed: (View Article at PubMed) PMID:17510246
DOI: Full-text: DOI:10.1210/en.2007-0029

Active thyroid hormones are critical for the differentiation and function of brown adipose tissue. However, we have observed high basal and induced levels of type 3 deiodinase (D3), an enzyme that inactivates thyroid hormones and is coded by the imprinted gene Dio3, in differentiating brown preadipocytes in primary culture. We find that D3 activity and mRNA expression strongly correlate with the rate of proliferation of undifferentiated precursor cells under various conditions. Furthermore, differentiation of precursor cells to adipocytes is associated with decreased levels of D3 expression, and only very low levels of D3 mRNA are found in mature adipocytes. Dlk1, an inhibitor of adipocyte differentiation and a paternally expressed gene located in the same imprinted domain as Dio3, displayed changes in expression that parallel those of Dio3. In contrast, a 4-kb transcript for Dio3os, an antisense gene also located in the same imprinted domain, is markedly up-regulated in differentiated adipocytes. We conclude that D3 expression in differentiating preadipocytes is primarily linked to proliferating cells, whereas Dio3os expression is associated with mature adipocytes. Our results suggest that genomic imprinting and gene expression at the Dlk1/Dio3 imprinted domain may play a role in the regulation of adipocyte proliferation and differentiation.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Dio3  (iodothyronine deiodinase 3)


Additional Information