RGD Reference Report - Kinetic studies of the reaction mechanism of rat liver phosphoglycerate kinase in the direction of ADP utilization. - Rat Genome Database

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Kinetic studies of the reaction mechanism of rat liver phosphoglycerate kinase in the direction of ADP utilization.

Authors: Lavoinne, A  Marchand, JC  Chedeville, A  Matray, F 
Citation: Lavoinne A, etal., Biochimie. 1983 Mar;65(3):211-20.
RGD ID: 2302859
Pubmed: PMID:6405813   (View Abstract at PubMed)

The kinetic properties of rat liver phosphoglycerate kinase were investigated in the forward direction of the reaction (utilization of ADP). The kinetic studies were performed in an assay system using combined hexokinase/glucose-6-phosphate dehydrogenase as an ATP trap. The Km values for Mg ADP1- and 1,3-diphospho-D-glycerate were approximately 0.11 and 0.006 mM, respectively. Reciprocal plots of 1/v versus 1/ (Mg ADP1-) at different fixed concentrations of 1,3-diphospho-D-glycerate and 1/v versus 1/ (1,3-diphospho-D-glycerate) at different fixed concentrations of Mg ADP1- were apparently parallel. However, product inhibition studies (3-phospho-D-glycerate), dead-end inhibition studies (2,3-diphospho-D-glycerate), and adenosine and AMP inhibition patterns yielded results consistent with a rapid equilibrium random mechanism in which the binding of one substrate greatly decreases the affinity of the enzyme for the second substrate. Existence of two sites for 3-phospho-D-glycerate is suggested.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glycolytic process  IDA 2302859 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ADP binding  IDA 2302859 RGD 
phosphoglycerate kinase activity  IDA 2302859 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glycolysis pathway   ISOPgk1 (Rattus norvegicus)2302859; 2302859 RGD 
glycolysis pathway   IDA 2302859 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Pgk1  (phosphoglycerate kinase 1)

Genes (Mus musculus)
Pgk1  (phosphoglycerate kinase 1)

Genes (Homo sapiens)
PGK1  (phosphoglycerate kinase 1)


Additional Information