RGD Reference Report - Proteomic identification of brain proteins that interact with dynein light chain LC8. - Rat Genome Database

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Proteomic identification of brain proteins that interact with dynein light chain LC8.

Authors: Navarro-Lerida, I  Martinez Moreno, M  Roncal, F  Gavilanes, F  Albar, JP  Rodriguez-Crespo, I 
Citation: Navarro-Lerida I, etal., Proteomics. 2004 Feb;4(2):339-46.
RGD ID: 2301546
Pubmed: PMID:14760703   (View Abstract at PubMed)
DOI: DOI:10.1002/pmic.200300528   (Journal Full-text)

Cytoplasmic dynein is a large minus end-directed microtubule motor that translocates cargos towards the minus end of microtubules. Light chain 8 of the dynein machinery (LC8) has been reported to interact with a large variety of proteins that possess K/RSTQT or GIQVD motifs in their sequence, hence permitting their transport in a retrograde manner. Yeast two-hybrid analysis has revealed that in brain, LC8 associates directly with several proteins such as neuronal nitric oxide synthase, guanylate kinase domain-associated protein and gephyrin. In this work, we report the identification of over 40 polypeptides, by means of a proteomic approach, that interact with LC8 either directly or indirectly. Many of the neuronal proteins that we identified cluster at the post-synaptic terminal, and some of them such as phosphofructokinase, lactate dehydrogenase or aldolase are directly involved in glutamate metabolism. Other pool of proteins identified displayed the LC8 consensus binding motif. Finally, recombinant LC8 was produced and a library of overlapping dodecapeptides (pepscan) was employed to map the LC8 binding site of some of the proteins that were previously identified using the proteomic approach, hence confirming binding to the consensus binding sites.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GlulRatdynein light chain binding  IPIDynll1 (Rattus norvegicus) RGD 
Dynll1Ratenzyme binding  IPIGlul (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dynll1  (dynein light chain LC8-type 1)
Glul  (glutamate-ammonia ligase)


Additional Information