RGD Reference Report - Alterations in EDHF-type relaxation and phosphodiesterase activity in mesenteric arteries from diabetic rats. - Rat Genome Database

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Alterations in EDHF-type relaxation and phosphodiesterase activity in mesenteric arteries from diabetic rats.

Authors: Matsumoto, T  Kobayashi, T  Kamata, K 
Citation: Matsumoto T, etal., Am J Physiol Heart Circ Physiol. 2003 Jul;285(1):H283-91.
RGD ID: 2300416
Pubmed: PMID:12793980   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpheart.00954.2002   (Journal Full-text)

In isolated superior mesenteric artery rings from age-matched control rats and streptozotocin (STZ)-induced diabetic rats, we investigated the role of cAMP in endothelium-derived hyperpolarizing factor (EDHF)-type relaxation. The ACh-induced EDHF-type relaxation was significantly weaker in STZ-induced diabetic rats than in control rats, and in both groups of rats it was attenuated by 18alpha-glycyrrhetinic acid (18alpha-GA), an inhibitor of gap junctions, and enhanced by IBMX, a cAMP-phosphodiesterase (PDE) inhibitor. These enhanced EDHF-type responses were very similar in magnitude between diabetic and age-matched control rats. The EDHF-type relaxation was enhanced by cilostamide, a PDE3-selective inhibitor, but not by Ro 20-1724, a PDE4-selective inhibitor. The expression levels of the mRNAs and proteins for two cAMP PDEs (PDE3A, PDE3B) were significantly increased in STZ-induced diabetic rats, but those for PDE4D were not. We conclude that the impairment of EDHF-type relaxations in STZ-induced diabetic rats may be attributed to a reduction in the action of cAMP via increased PDE activity.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PDE3AHumanExperimental Diabetes Mellitus  ISOPde3a (Rattus norvegicus)mRNA and protein:increased expression:mesenteric arteryRGD 
Pde3aRatExperimental Diabetes Mellitus  IEP mRNA and protein:increased expression:mesenteric arteryRGD 
Pde3aMouseExperimental Diabetes Mellitus  ISOPde3a (Rattus norvegicus)mRNA and protein:increased expression:mesenteric arteryRGD 

Objects Annotated

Genes (Rattus norvegicus)
Pde3a  (phosphodiesterase 3A)

Genes (Mus musculus)
Pde3a  (phosphodiesterase 3A, cGMP inhibited)

Genes (Homo sapiens)
PDE3A  (phosphodiesterase 3A)


Additional Information