RGD Reference Report - [Expression of NF-kappa B in human bladder cancer and its clinical significance] - Rat Genome Database

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[Expression of NF-kappa B in human bladder cancer and its clinical significance]

Authors: Xie, DH  Tang, XD  Xia, SJ  Tan, JM  Wang, XH  Cai, Y 
Citation: Xie DH, etal., Ai Zheng. 2002 Jun;21(6):663-7.
RGD ID: 2300270
Pubmed: PMID:12452071   (View Abstract at PubMed)

BACKGROUND & OBJECTIVE: Studies on solid cancer(such as breast cancer, hepatocellular cancer, pancreatic cancer) indicated that the abnormal expression of nuclear transcription factor Kappa B (NF-kappa B) regulates angiogenesis and cyclin-related genes. This study was designed to investigate the expression differences of NF-kappa B and its regulated genes between human primary transitional cell carcinoma(TCCs) of bladder and non-cancer bladder mucosa and its clinical significance. METHODS: Forty-three frozen sections including 30 bladder cancer and 13 non-cancer bladder mucosa were subjected to immunohistochemistry and nucleus staining for determining levels of NF-kappa B family and I kappa B alpha; Five paired cancer and non-cancer specimens were subjected to Western blot for analysis p65, an important subtype of NF-kappa B; Thirteen paired specimens were subjected to RT-PCR for determination mRNA levels of p50, p52, p65, c-Rel, RelB, I kappa B alpha, CyclinD1, IL-8. RESULTS: Expressions of p50, p52, p65, c-Rel, RelB, I kappa B alpha, CyclinD1, IL-8 mRNAs in bladder cancer were higher than that in non-cancer bladder mucosa (P < 0.01, P < 0.05, P < 0.01, P < 0.01, P < 0.05, P < 0.05, P < 0.05 and P < 0.05, respectively). Nucelus stainings of p50, p52, p65, c-Rel, RelB were also stronger in bladder cancer(P < 0.01, P < 0.01, P < 0.01, P < 0.01 and P < 0.01, respectively). Moreover, p65 was expressed more in cancer tissue than that in non-cancer mucosa evidenced by Western blot. In bladder cancer, the ranking score differences of p52, p65, c-Rel protein between lymphatic metastasis group and non-lymphatic metastasis group were statistically significant (P < 0.01, P < 0.01, P < 0.05, respectively). CONCLUSIONS: Compared to noncancer bladder mucosa, expressions of NF-kappa B family and its regulated genes in bladder cancer are markedly higher. NF-kappa B may be related to lymphatic metastasis.

RGD Manual Disease Annotations    Click to see Annotation Detail View

Objects Annotated

Genes (Rattus norvegicus)
Rel  (REL proto-oncogene, NF-kB subunit)
Relb  (RELB proto-oncogene, NF-kB subunit)

Genes (Mus musculus)
Rel  (reticuloendotheliosis oncogene)
Relb  (avian reticuloendotheliosis viral (v-rel) oncogene related B)

Genes (Homo sapiens)
REL  (REL proto-oncogene, NF-kB subunit)
RELB  (RELB proto-oncogene, NF-kB subunit)


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