RGD Reference Report - The isolation of eukaryotic ribosomal proteins. The purification and characterization of the 40 S ribosomal subunit proteins S2, S3, S4, S5, S6, S7, S8, S9, S13, S23/S24, S27, and S28. - Rat Genome Database

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The isolation of eukaryotic ribosomal proteins. The purification and characterization of the 40 S ribosomal subunit proteins S2, S3, S4, S5, S6, S7, S8, S9, S13, S23/S24, S27, and S28.

Authors: Collatz, E  Wool, IG  Lin, A  Stoffler, G 
Citation: Collatz E, etal., J Biol Chem. 1976 Aug 10;251(15):4666-72.
RGD ID: 2300010
Pubmed: PMID:947902   (View Abstract at PubMed)

The proteins of the small subunit of rat liver ribosomes were separated into five groups by stepwise elution from carboxymethylcellulose with LiCl at pH 6.5 (Collatz, E., Lin, A., Stoffler, G., Tsurugi, K., and Wool, I.G., (1976) J. Biol. Chem. 251, 1808-1816). From the several groups, 12 proteins (S2,S3, S4, S5, S6, S7, S8, S9, S13, S23/S24, S27, and S28) wereisolated by ion exchange chromatography on carboxymethylcellulose, by chromatography on sulfopropyl-Sephadex, and by gel filtration through Sephadex G-75. The amount of protein obtained varied from 1 to 9 mg depending on the number of steps required for the preparation; several proteins had no detectable contamination and the impurities in the others were no greater than 9%. The molecular weight of the proteins was estimated by polyazrylamide gel electrophoresis in sodium dodecyl sulfate; the amino acid composition was determined.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  

Objects Annotated

Genes (Rattus norvegicus)
Rps13  (ribosomal protein S13)
Rps2  (ribosomal protein S2)
Rps27  (ribosomal protein S27)
Rps28  (ribosomal protein S28)
Rps3  (ribosomal protein S3)
Rps5  (ribosomal protein S5)
Rps6  (ribosomal protein S6)
Rps7  (ribosomal protein S7)
Rps8  (ribosomal protein S8)
Rps9  (ribosomal protein S9)


Additional Information