RGD Reference Report - Zhonghua zhong liu za zhi [Chinese journal of oncology] - Rat Genome Database

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Zhonghua zhong liu za zhi [Chinese journal of oncology]

Authors: Fan, Z  Zhang, J  Bai, J 
Citation: Fan Z, etal., Zhonghua Zhong Liu Za Zhi. 2001 Mar;23(2):135-7.
RGD ID: 2299972
Pubmed: PMID:11783019   (View Abstract at PubMed)

OBJECTIVE: To investigate the role of loss of heterozygosity (LOH) on chromosome 18q in the carcinogenesis and progression of urinary bladder cancer and to provide clues for early detection and positional cloning of related genes. METHODS: Deletion mapping was performed on 18q using 12 microsatellite markers. Immunohistochemistry staining was used to evaluate the expression level of two tumor suppressor candidates Smad 2 and Smad 4 in this region. RESULTS: LOH in at least one of the 12 microsatellites on 18q was detected in 84.2% (32/38) of patients with bladder cancer. The minimal deletion region included tumor suppressor candidate Smad 4. Immunohistochemical study revealed that 21.1% (8/38) of the cases had up-regulated Smad 2 protein and 34.2% (13/38) of the cases had down-regulated Smad 2 protein. Smad4 protein expression was down-regulated in 68.4% (26/38) of the cases but up-regulated Smad 4 expression was seen in only one case. CONCLUSIONS: LOH on 18q was closely linked with bladder cancer. The irregular expression of tumor suppressor candidates Smad 2 and Smad 4 may play important role in the initiation and progression of bladder neoplasms.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SMAD4Humanurinary bladder cancer  IEP protein:decreased expression:urinary bladderRGD 
Smad4Raturinary bladder cancer  ISOSMAD4 (Homo sapiens)protein:decreased expression:urinary bladderRGD 
Smad4Mouseurinary bladder cancer  ISOSMAD4 (Homo sapiens)protein:decreased expression:urinary bladderRGD 

Objects Annotated

Genes (Rattus norvegicus)
Smad4  (SMAD family member 4)

Genes (Mus musculus)
Smad4  (SMAD family member 4)

Genes (Homo sapiens)
SMAD4  (SMAD family member 4)


Additional Information