RGD Reference Report - Large-scale purification and further characterization of rat pristanoyl-CoA oxidase. - Rat Genome Database

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Large-scale purification and further characterization of rat pristanoyl-CoA oxidase.

Authors: Van Veldhoven, PP  Van Rompuy, P  Fransen, M  De Bethune, B  Mannaerts, GP 
Citation: Van Veldhoven PP, etal., Eur J Biochem. 1994 Jun 15;222(3):795-801.
RGD ID: 2299969
Pubmed: PMID:8026493   (View Abstract at PubMed)

The elution of pristanoyl-CoA oxidase from butyl-Sepharose required unusually high concentrations of ethylene glycol, enabling the large-scale purification of this oxidase in a single chromatographic step. The enzyme, the native molecular mass of which was estimated previously at 415 kDa by gel filtration (Van Veldhoven, P.P., Vanhove, G., Vanhoutte, F., Dacremont, G., Eyssen, H. J. & Mannaerts, G. P. (1991) J. Biol. Chem. 266, 24676-24683), migrated as a 513-kDa protein during native gel electrophoresis. It showed a typical flavoprotein spectrum and probably binds 4 mol FAD/mol enzyme. Its amino acid composition was different from those of other known acyl-CoA oxidases. Screening of different rat tissues, either for enzyme activity or by immunoblotting, revealed the highest level of pristanoyl-CoA oxidase in liver, followed by kidney, intestinal mucosa, spleen and lung. The oxidase activities, measured with 2-methylpalmitoyl-CoA as the substrate, in livers from other vertebrates including man were low compared to rat. This was also confirmed by immunoblotting which provided a clear signal only in rat liver, possibly indicating that pristanoyl-CoA oxidase might be a rat-specific oxidase.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Acox3Ratfatty acid beta-oxidation using acyl-CoA oxidase  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Acox3Ratfatty acid binding  IDA  RGD 
Acox3Ratflavin adenine dinucleotide binding  IDA  RGD 
Acox3Ratpristanoyl-CoA oxidase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Acox3  (acyl-CoA oxidase 3, pristanoyl)


Additional Information