RGD Reference Report - LRP: a new adhesion molecule for endothelial and smooth muscle cells. - Rat Genome Database

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LRP: a new adhesion molecule for endothelial and smooth muscle cells.

Authors: Hu, C  Oliver, JA  Goldberg, MR  Al-Awqati, Q 
Citation: Hu C, etal., Am J Physiol Renal Physiol. 2001 Oct;281(4):F739-50.
RGD ID: 2299067
Pubmed: PMID:11553521   (View Abstract at PubMed)

We recently generated a monoclonal antibody that disrupted the association of endothelial cells with their target location during kidney development. Here, we purified the antigen of this monoclonal antibody to homogeneity using rat mesangial cell cytosol. Sequence revealed that it is a previously identified protein, termed the "laminin receptor precursor" (LRP). We found that this protein is expressed in most tissues, but immunocytochemistry revealed that it is present largely or entirely in blood vessels where it is located underneath endothelial cells and in between smooth muscle cells of the vascular wall. Vascular smooth muscle cells such as mesangial cells produce and secrete LRP into their extracellular matrix where it is present in several molecular weight forms. Endothelial cells produce very little if any of the protein, but they bind avidly to LRP-coated dishes. Anti-LRP antibodies prevent the binding of smooth muscle cells to uncoated plates, implying that cells that secrete it use it for attachment. In an assay for heterologous cell-to-cell interaction, antibodies to LRP inhibited the binding of smooth muscle cells to endothelial cells. Maturation and differentiation of blood vessels require interaction between endothelial and smooth muscle cells. LRP is a new component of the mesangial matrix, and we propose that it is an adhesion molecule that mediates an interaction between smooth muscle cells and endothelia.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell-cell adhesion  IDA 2299067 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
basement membrane  IDA 2299067 RGD 
collagen-containing extracellular matrix  IDA 2299067 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
structural molecule activity  ICGO:00163372299067 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rpsa  (ribosomal protein SA)


Additional Information