RGD Reference Report - Alterations of the metastasis suppressor gene nm23 and the proto-oncogene c-myc in human testicular germ cell tumors. - Rat Genome Database

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Alterations of the metastasis suppressor gene nm23 and the proto-oncogene c-myc in human testicular germ cell tumors.

Authors: Schmidt, B  Ackermann, R  Hartmann, M  Strohmeyer, T 
Citation: Schmidt B, etal., J Urol. 1997 Nov;158(5):2000-5.
RGD ID: 2299060
Pubmed: PMID:9334657   (View Abstract at PubMed)

The putative metastasis suppressor genes nm23-H1, nm23-H2 and the c-myc proto-oncogene were investigated in testicular germ cell tumors (GCTs) using Southern and Northern blotting as well as semiquantitative reverse transcription polymerase chain reaction (RT-PCR) and single strand conformation polymorphism (SSCP) analysis. When studying Bgl II RFLPs, allelic losses of the nm23 gene were found in 3/12 (25%) informative tumors, and all 3 had lymph node and/or distant metastases. A 2 to 7 fold nm23 mRNA overexpression was found in 22/34 (64.7%) tumors examined. RT-PCR revealed that this phenomenon is mainly a consequence of nm23-H2 overexpression. Overexpression of both the H1 and the H2 gene was predominantly found in the seminoma subtype and was not associated with tumor stage. Only 1/25 tumors, a seminoma with distant metastases, had a point mutation in the coding region of the nm23-H2 gene as demonstrated by SSCP analysis. None of the 8 seminomas and only 1/13 non-seminomas had c-myc overexpression. No abnormalities of the c-myc gene could be detected on the DNA level. Despite the fact that in previous investigations nm23-H2 was demonstrated to be a putative transcription factor for c-myc, no coexpression of c-myc and nm23-H2 was found by quantitative RT-PCR in this study.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Germ Cell and Embryonal Neoplasms  IEP 2299060mRNA:increased expression:testisRGD 
Germ Cell and Embryonal Neoplasms  ISONME2 (Homo sapiens)2299060; 2299060mRNA:increased expression:testisRGD 

Objects Annotated

Genes (Rattus norvegicus)
Nme2  (NME/NM23 nucleoside diphosphate kinase 2)

Genes (Mus musculus)
Nme2  (NME/NM23 nucleoside diphosphate kinase 2)

Genes (Homo sapiens)
NME2  (NME/NM23 nucleoside diphosphate kinase 2)


Additional Information