RGD Reference Report - Isolation of the cytochrome-bc1 complex from rat-liver mitochondria. - Rat Genome Database

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Isolation of the cytochrome-bc1 complex from rat-liver mitochondria.

Authors: Gellerfors, P  Johansson, T  Nelson, BD 
Citation: Gellerfors P, etal., Eur J Biochem. 1981 Apr;115(2):275-8.
RGD ID: 2298982
Pubmed: PMID:6263624   (View Abstract at PubMed)

The cytochrome bc1 complex has been isolated from rat-liver mitochondria by two different procedures. The enzyme isolated by either procedure exhibits a specific cytochrome b and cytochrome c1 heme content of approximately 8 and 4 nmol/mg protein respectively. Both preparations contain only seven polypeptides on sodium dodecylsulfate gel electrophoresis, with the following apparent molecular weights: I, 50000; II, 46000; III, 33000; IV, 25000; V, 12500; VI, 10000; VII, 5600. The polypeptide composition is identical to that of the beef-heart enzyme isolated by cholate/ammonium sulfate fractionation. Furthermore, with the exception of subunit II (core protein 2), the apparent molecular weights of the subunits are identical in the rat-liver and beef-heart enzymes.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
mitochondrial inner membrane  IDA 2298982 RGD 
protein-containing complex  IDA 2298982 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex binding  IPICyc1 (Rattus norvegicus)2298982 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mt-cyb  (mitochondrially encoded cytochrome b)


Additional Information