RGD Reference Report - Hypoosmolarity influences the activity of transcription factor NF-kappaB in rat H4IIE hepatoma cells. - Rat Genome Database

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Hypoosmolarity influences the activity of transcription factor NF-kappaB in rat H4IIE hepatoma cells.

Authors: Michalke, M  Cariers, A  Schliess, F  Haussinger, D 
Citation: Michalke M, etal., FEBS Lett. 2000 Jan 7;465(1):64-8.
RGD ID: 2298882
Pubmed: (View Article at PubMed) PMID:10620707

The influence of anisoosmolarity on NF-kappaB binding activity was studied in H4IIE rat hepatoma cells. Hypoosmolarity induced a sustained NF-kappaB binding activity whereas the hyperosmotic NF-kappaB response was only minor. Hypoosmotic NF-kappaB activation was accompanied by degradation of the inhibitory IkappaB-alpha. Protein kinase C, PI(3)-kinase, reactive oxygen intermediates and the proteasome apparently participate in mediating the hypoosmotic effect on NF-kappaB. Hypoosmolarity plus PMA induced, amplified and prolonged IkappaB-alpha degradation and NF-kappaB binding activity. Transforming growth factor beta-induced apoptosis was diminished by hypoosmolarity. However, this anti-apoptotic effect was probably not related to NF-kappaB activation.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Nfkb1  (nuclear factor kappa B subunit 1)
Nfkbia  (NFKB inhibitor alpha)
Rela  (RELA proto-oncogene, NF-kB subunit)


Additional Information