RGD Reference Report - Acetaldehyde inhibits NF-kappaB activation through IkappaBalpha preservation in rat Kupffer cells. - Rat Genome Database

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Acetaldehyde inhibits NF-kappaB activation through IkappaBalpha preservation in rat Kupffer cells.

Authors: Jokelainen, K  Thomas, P  Lindros, K  Nanji, AA 
Citation: Jokelainen K, etal., Biochem Biophys Res Commun. 1998 Dec 30;253(3):834-6.
RGD ID: 2298880
Pubmed: (View Article at PubMed) PMID:9918814
DOI: Full-text: DOI:10.1006/bbrc.1998.9863

BACKGROUND AND AIMS: Treatment with acetaldehyde dehydrogenase inhibitors leads to increased liver acetaldehyde levels and prevents hepatic inflammation and necrosis in ethanol-fed rats. This is accompanied by IkappaBa preservation and decreased activation of nuclear factor (NF)-kappaB. The present in vitro study was aimed to clarify whether acetaldehyde has an effect on degradation of IkappaBalpha and activation of NF-kappaB in LPS-stimulated rat Kupffer cells. METHODS: Kupffer cells were isolated from male Sprague-Dawley rats and preincubated with various concentrations of acetaldehyde (25-100 microM). Thereafter the cells were stimulated with LPS, and cytosolic and nuclear fractions were prepared. IkappaBalpha and p65 proteins and activation of NF-kappaB were evaluated. RESULTS: In LPS-stimulated rat Kupffer cells, acetaldehyde diminished proteolytic degradation of IkappaBalpha, inhibited nuclear translocation of cytosolic p65 protein, and, accordingly, markedly decreased NF-kappaB activation. CONCLUSIONS: Acetaldehyde is clearly involved in the stabilization of IkappaBalpha protein and suppression of NF-kappaB activation in rat Kupffer cells. Acetaldehyde may form an adduct with IkappaBalpha, thus making the protein less susceptible to degradation.


Gene Ontology Annotations    

Biological Process

Cellular Component
cytosol  (IDA)
nucleus  (IDA)

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Nfkbia  (NFKB inhibitor alpha)
Rela  (RELA proto-oncogene, NF-kB subunit)

Additional Information