RGD Reference Report - Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP. - Rat Genome Database

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Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP.

Authors: Maita, N  Okada, K  Hatakeyama, K  Hakoshima, T 
Citation: Maita N, etal., Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1212-7. Epub 2002 Jan 29.
RGD ID: 2298660
Pubmed: PMID:11818540   (View Abstract at PubMed)
PMCID: PMC122169   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.022646999   (Journal Full-text)

In the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin. The crystal structure of the stimulatory complex reveals that the GTPCHI decamer is sandwiched by two GFRP homopentamers. Each GFRP pentamer forms a symmetrical five-membered ring similar to beta-propeller. Five phenylalanine molecules are buried inside each interface between GFRP and GTPCHI, thus enhancing the binding of these proteins. The complex structure suggests that phenylalanine-induced GTPCHI x GFRP complex formation enhances GTPCHI activity by locking the enzyme in the active state.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex assembly  IDA 2298660; 2298660 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex  IDA 2298660; 2298660 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
amino acid binding  IDA 2298660 RGD 
protein binding enablesIPIUniProtKB:P705522298660PMID:11818540IntAct 
protein binding enablesIPIUniProtKB:P222882298660PMID:11818540IntAct 
protein-containing complex binding  IDA 2298660; 2298660heterooligomerizationRGD 
zinc ion binding  IDA 2298660 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gch1  (GTP cyclohydrolase 1)
Gchfr  (GTP cyclohydrolase I feedback regulator)


Additional Information