RGD Reference Report - Expression of platelet-derived growth factor-A (PDGF-A), PDGF-B, and PDGF receptor-alpha and -beta during human testicular development and disease. - Rat Genome Database

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Expression of platelet-derived growth factor-A (PDGF-A), PDGF-B, and PDGF receptor-alpha and -beta during human testicular development and disease.

Authors: Basciani, S  Mariani, S  Arizzi, M  Ulisse, S  Rucci, N  Jannini, EA  Della Rocca, C  Manicone, A  Carani, C  Spera, G  Gnessi, L 
Citation: Basciani S, etal., J Clin Endocrinol Metab. 2002 May;87(5):2310-9.
RGD ID: 2298579
Pubmed: PMID:11994382   (View Abstract at PubMed)
DOI: DOI:10.1210/jcem.87.5.8476   (Journal Full-text)

Platelet-derived growth factor (PDGF) plays a critical role in regulating the development and functional control of various tissues and has been implicated in the pathogenesis of serious diseases, including cancer and atherosclerosis. Given the emerging role of PDGF in the development and function of male gonads, we compared the expression profiles of the mRNAs of the PDGF A- and B-chains and of the PDGF receptor (PDGFR) alpha- and beta-subunits in fetal and adult human testis. The immunohistochemical localization of the corresponding proteins in fetal, adult, and diseased human testicular tissues was also analyzed. PDGFs and PDGFRs mRNAs were readily detected by both Northern analysis and RT-PCR. The transcript levels were higher during 16-20 wk gestation, significantly lower at 24-28 wk, and increased in the adult. An identical pattern of protein expression was confirmed by immunohistochemistry, although the cellular localization of the PDGF system changes during postnatal development, concomitantly with the progression of spermatogenesis. In the testicular samples from patients affected by either complete aplasia of germ cells or various grades of spermatogenic arrest, the immunohistochemical localization of PDGFs and PDGFRs was different from normal, confirming a close connection between germ cells and PDGF system distribution. These results indicate that PDGF, through complex interactions, could play a leading role in ontogenesis and testicular pathophysiology in humans. Finally, the expression of PDGF ligands and receptor proteins in Leydig cell tumors suggests a relationship of the PDGF system to tumorigenesis or tumor progression in this testicular neoplasm.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PDGFAHumanLeydig cell tumor  IEP protein:increased expression:testis and leydig cellRGD 
PDGFBHumanLeydig cell tumor  IEP protein:increased expression:testis and leydig cellRGD 
PDGFRAHumanLeydig cell tumor  IEP protein:increased expression:testis and leydig cellRGD 
PDGFRBHumanLeydig cell tumor  IEP protein:increased expression:testis and leydig cellRGD 
PdgfaRatLeydig cell tumor  ISOPDGFA (Homo sapiens)protein:increased expression:testis and leydig cellRGD 
PdgfaMouseLeydig cell tumor  ISOPDGFA (Homo sapiens)protein:increased expression:testis and leydig cellRGD 
PdgfbRatLeydig cell tumor  ISOPDGFB (Homo sapiens)protein:increased expression:testis and leydig cellRGD 
PdgfbMouseLeydig cell tumor  ISOPDGFB (Homo sapiens)protein:increased expression:testis and leydig cellRGD 
PdgfraRatLeydig cell tumor  ISOPDGFRA (Homo sapiens)protein:increased expression:testis and leydig cellRGD 
PdgfraMouseLeydig cell tumor  ISOPDGFRA (Homo sapiens)protein:increased expression:testis and leydig cellRGD 
PdgfrbRatLeydig cell tumor  ISOPDGFRB (Homo sapiens)protein:increased expression:testis and leydig cellRGD 
PdgfrbMouseLeydig cell tumor  ISOPDGFRB (Homo sapiens)protein:increased expression:testis and leydig cellRGD 

Objects Annotated

Genes (Rattus norvegicus)
Pdgfa  (platelet derived growth factor subunit A)
Pdgfb  (platelet derived growth factor subunit B)
Pdgfra  (platelet derived growth factor receptor alpha)
Pdgfrb  (platelet derived growth factor receptor beta)

Genes (Mus musculus)
Pdgfa  (platelet derived growth factor, alpha)
Pdgfb  (platelet derived growth factor, B polypeptide)
Pdgfra  (platelet derived growth factor receptor, alpha polypeptide)
Pdgfrb  (platelet derived growth factor receptor, beta polypeptide)

Genes (Homo sapiens)
PDGFA  (platelet derived growth factor subunit A)
PDGFB  (platelet derived growth factor subunit B)
PDGFRA  (platelet derived growth factor receptor alpha)
PDGFRB  (platelet derived growth factor receptor beta)


Additional Information