RGD Reference Report - Delayed activation and regulation of MKK7 in hippocampal CA1 region following global cerebral ischemia in rats. - Rat Genome Database

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Delayed activation and regulation of MKK7 in hippocampal CA1 region following global cerebral ischemia in rats.

Authors: Zhang, Q  Tian, H  Fu, X  Zhang, G 
Citation: Zhang Q, etal., Life Sci. 2003 Nov 21;74(1):37-45.
RGD ID: 2298566
Pubmed: PMID:14575811   (View Abstract at PubMed)

c-Jun N-terminal protein kinase (JNK) activation and subsequent c-Jun phosphorylation which stimulates its transcriptional activity have been well studied in cerebral ischemia. To determine whether mitogen-activated protein kinase kinase 7 (MKK7) play a role in JNK activation in response to the stress of global cerebral ischemia, we tested the activation of such a kinase by using phospho-Ser and phospho-Thr antibodies. Immunoprecipitation and Western blot analysis revealed that MKK7 was expressed at similar levels in all conditions, whereas phospho-MKK7 was highly augmented from 1 to 5 days and reached its peak at 3 days after 15 min of ischemia. Consistent with the active phase, the interaction of MLK3, ASK1 and phospho-JNK with MKK7 was increased compared with sham control, as shown by coimmunoprecipitation experiments. Moreover, MKK7 activation was markedly reduced by pretreatment of the free radical scavenging thiol antioxidant N-acetylcysteine (NAC). Together with previous studies, the late activation of MKK7 in hippocampal CA1 region may contribute to delayed cell death, and the protective effects of antioxidant against ischemia-induced injury may be partially mediated by the down-regulation of JNK signal pathway.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Map2k7  (mitogen activated protein kinase kinase 7)
Map3k11  (mitogen-activated protein kinase kinase kinase 11)


Additional Information