RGD Reference Report - Multiple gene expression analyses in paraffin-embedded tissues by TaqMan low-density array: Application to hedgehog and Wnt pathway analysis in ovarian endometrioid adenocarcinoma.

General

Multiple gene expression analyses in paraffin-embedded tissues by TaqMan low-density array: Application to hedgehog and Wnt pathway analysis in ovarian endometrioid adenocarcinoma.
Authors:	Steg, A Wang, W Blanquicett, C Grunda, JM Eltoum, IA Wang, K Buchsbaum, DJ Vickers, SM Russo, S Diasio, RB Frost, AR LoBuglio, AF Grizzle, WE Johnson, MR
Citation:	Steg A, etal., J Mol Diagn. 2006 Feb;8(1):76-83.
RGD ID:	2298535
Pubmed:	PMID:16436637 (View Abstract at PubMed)
PMCID:	PMC1867577 (View Article at PubMed Central)
DOI:	DOI:10.2353/jmoldx.2006.040402 (Journal Full-text)
Recent studies have shown the hedgehog and Wnt families of signaling proteins to be associated with tumor initiation, growth, and survival. However, these pathways remain unexplored in ovarian endometrioid adenocarcinoma (OEA). Here, we describe a novel TaqMan low-density array to examine the expression of 26 and 20 genes in the hedgehog and Wnt pathways, respectively, in six matched snap-frozen and formalin-fixed, paraffin-embedded (FPE) OEA specimens. Expression values were normalized to uninvolved ovarian epithelium. Gene expression in matched frozen and FPE tissues demonstrated significant concordance (r = 0.92, P < 0.0001). However, comparison of amplified and unamplified RNA from frozen OEA tissues revealed an altered molecular profile in amplified RNA. Amplification of RNA from FPE tissues was not successful. The expression of Desert hedgehog (DHH), Indian hedgehog (IHH), Hedge-hog interacting protein (HHIP), Wnt10B, Wnt9B, and Wnt inhibitory factor (WIF1) were tumor-specific with no detectable expression in normal ovarian epithelium. In addition, several genes were significantly (P < 0.025) down-regulated in OEA, including cyclin E2, Porcupine, c-Myc, and Axin 2 (4.8-, 3.6-, 2.9-, and 1.9-fold, respectively). TaqMan low-density array provides an effective multivariate technique for examining gene expression in RNA isolated from either snap-frozen or archival FPE tissues and can identify tumor-specific genes, possibly leading to novel treatments.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
Endometrioid Carcinomas		IEP		2298535	mRNA:increased expression:ovary	RGD
Endometrioid Carcinomas		ISO	WIF1 (Homo sapiens)	2298535; 2298535	mRNA:increased expression:ovary	RGD

Objects Annotated

Genes (Rattus norvegicus)

Wif1 (Wnt inhibitory factor 1)

Genes (Mus musculus)

Wif1 (Wnt inhibitory factor 1)

Genes (Homo sapiens)

WIF1 (WNT inhibitory factor 1)

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Multiple gene expression analyses in paraffin-embedded tissues by TaqMan low-density array: Application to hedgehog and Wnt pathway analysis in ovarian endometrioid adenocarcinoma.