RGD Reference Report - Evidence of a role for the INK4 family of cyclin-dependent kinase inhibitors in ovarian granulosa cell tumors. - Rat Genome Database

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Evidence of a role for the INK4 family of cyclin-dependent kinase inhibitors in ovarian granulosa cell tumors.

Authors: Arcellana-Panlilio, MY  Egeler, RM  Ujack, E  Magliocco, A  Stuart, GC  Robbins, SM  Coppes, MJ 
Citation: Arcellana-Panlilio MY, etal., Genes Chromosomes Cancer. 2002 Oct;35(2):176-81.
RGD ID: 2296066
Pubmed: PMID:12203782   (View Abstract at PubMed)
DOI: DOI:10.1002/gcc.10108   (Journal Full-text)

Granulosa cell tumors (GCTs) of the ovary are relatively rare and account for <5% of all ovarian cancers. The molecular pathogenesis of these tumors is not well understood. We tested the hypothesis that cyclin-dependent kinase inhibitors, specifically the inhibitors of the cyclin-dependent kinase 4 (INK4) family, are targets for altered gene expression in GCTs. The status of RB1, INK4A, INK4B, INK4C, INK4D, and ARF in 13 adult and 2 juvenile ovarian GCTs was determined by reverse transcription-polymerase chain reaction of total RNA and exon-specific sequencing of genomic DNA. Tumors showing loss of INK4A expression were assayed further by exon-deletion analysis and methylation-specific PCR. None of the juvenile tumors demonstrated altered expression, but 7/12 (58%) adult GCTs lacked expression of INK4A, INK4B, or both. In one of these cases, we noted a homozygous deletion of the INK4A locus, and in the remaining tumors we found hypermethylation of the promoter region, a mechanism that can lead to gene inactivation. These data support a role for the INK4 family of CDK inhibitors in the biology of GCTs.

RGD Manual Disease Annotations    Click to see Annotation Detail View

Objects Annotated

Genes (Rattus norvegicus)
Cdkn2a  (cyclin-dependent kinase inhibitor 2A)
Cdkn2b  (cyclin-dependent kinase inhibitor 2B)

Genes (Mus musculus)
Cdkn2a  (cyclin dependent kinase inhibitor 2A)
Cdkn2b  (cyclin dependent kinase inhibitor 2B)

Genes (Homo sapiens)
CDKN2A  (cyclin dependent kinase inhibitor 2A)
CDKN2B  (cyclin dependent kinase inhibitor 2B)


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