RGD Reference Report - Differential Immunoreactivity of p16 in leiomyosarcomas and leiomyoma variants. - Rat Genome Database

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Differential Immunoreactivity of p16 in leiomyosarcomas and leiomyoma variants.

Authors: Gannon, BR  Manduch, M  Childs, TJ 
Citation: Gannon BR, etal., Int J Gynecol Pathol. 2008 Jan;27(1):68-73.
RGD ID: 2296049
Pubmed: PMID:18156978   (View Abstract at PubMed)
DOI: DOI:10.1097/pgp.0b013e3180ca954f   (Journal Full-text)

Several studies have now examined the cDNA expression profiles of healthy myometrium, leiomyomas (LM), and leiomyosarcomas (LMS). This has produced a list of candidate genes that might be useful tools for distinguishing these entities from each other. The potential candidates identified from this body of research include insulinlike growth factor 1, h-caldesmon, cytokeratin 18, and the cyclin-dependent kinase 4 inhibitor, p16. To determine whether the immunohistochemical expression of these proteins could aid in the diagnosis of LMS and LM variants, we constructed a tissue microarray consisting of cases of healthy myometrium (n = 10), LM (not otherwise specified and variants; n = 47), and LMS (n = 8), and then measured the immunoreactivity of each of these proteins. The cases were scored on the basis of staining intensity (weak, moderate, or strong) and extent (focal or diffuse) and were assigned a final score from 0 to +3. Immunostaining for p16 was statistically stronger in LMS than in LM and its subtypes (P < 0.001). Specifically, the p16 immunostaining score in LMS cases (n = 8) was at least +2, whereas the p16 immunostaining scores in all LM cases (n = 47) were either 0 (n = 35) or +1 (n = 12). The expression of the remaining antibodies did not show a statistically significant difference between the 2 groups. Furthermore, none of the markers studied showed any differences among the LM variants. The results of this study confirm the overexpression of p16 in LMS and suggest that p16 can serve as a reliable immunohistochemical marker in distinguishing uterine LMS from LM and its benign variants.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
leiomyosarcoma  IEP 2296049associated with Myometrial Neoplasms and protein:increased expression:myometriumRGD 
leiomyosarcoma  ISOCDKN2A (Homo sapiens)2296049; 2296049associated with Myometrial Neoplasms and protein:increased expression:myometriumRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cdkn2a  (cyclin-dependent kinase inhibitor 2A)

Genes (Mus musculus)
Cdkn2a  (cyclin dependent kinase inhibitor 2A)

Genes (Homo sapiens)
CDKN2A  (cyclin dependent kinase inhibitor 2A)


Additional Information