RGD Reference Report - Gene Expression Study of Aurora-A Reveals Implication During Bladder Carcinogenesis and Increasing Values in Invasive Urothelial Cancer. - Rat Genome Database

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Gene Expression Study of Aurora-A Reveals Implication During Bladder Carcinogenesis and Increasing Values in Invasive Urothelial Cancer.

Authors: Comperat, E  Bieche, I  Dargere, D  Laurendeau, I  Vieillefond, A  Benoit, G  Vidaud, M  Camparo, P  Capron, F  Verret, C  Cussenot, O  Bedossa, P  Paradis, V 
Citation: Comperat E, etal., Urology. 2008 May 14;.
RGD ID: 2293871
Pubmed: PMID:18485461   (View Abstract at PubMed)
DOI: DOI:10.1016/j.urology.2007.12.026   (Journal Full-text)

OBJECTIVES: Urothelial carcinoma is a frequent and aggressive cancer. We wanted to gain better insight into the early molecular mechanisms of bladder carcinogenesis by evaluating Aurora-A gene expression, which is implicated in genomic stability and essential for mitosis. MATERIALS: This study, using real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), analyzed the expression levels of three selected genes in dissected tissues from normal bladder, noninvasive cancers, and muscle-invasive bladder carcinomas (n = 49). We compared gene expression levels of three genes (Aurora-A, and as control uroplakin II (UPII) and TBP, respectively) at different stages of bladder cancer. We used multivariate analysis, receiver operating characteristic curves and the nonparametric Mann-Whitney test. RESULTS: The expression of Aurora-A gene studied was significantly deregulated, with an increasing level in cancer versus normal tissue Aurora-A. This development was linear. Aurora-A was already deregulated in early stages of carcinogenesis (pTa/pT1) (P = 0.0004) and displayed even more deregulation in muscle-invasive stages (pT2 to pT4). Immunohistochemistry performed on the same samples using Aurora-A antibody confirmed results of RT-PCR, with statistically significant values when comparing m-RNA expression and immunohistochemical values (P = 0.0001). CONCLUSIONS: This study highlights the fact that Aurora-A gene expression is already strongly deregulated in early stages of urothelial carcinoma with abnormal expression, and might be considered a biomarker of tumor aggression. The increase in Aurora-A expression might provide further information regarding the behavior of bladder cancer in daily practice.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AURKAHumanurinary bladder cancer disease_progressionIEP mRNA and protein:increased expression:urinary bladderRGD 
AurkaRaturinary bladder cancer disease_progressionISOAURKA (Homo sapiens)mRNA and protein:increased expression:urinary bladderRGD 
AurkaMouseurinary bladder cancer disease_progressionISOAURKA (Homo sapiens)mRNA and protein:increased expression:urinary bladderRGD 

Objects Annotated

Genes (Rattus norvegicus)
Aurka  (aurora kinase A)

Genes (Mus musculus)
Aurka  (aurora kinase A)

Genes (Homo sapiens)
AURKA  (aurora kinase A)


Additional Information