RGD Reference Report - Neoplastic and non-neoplastic cell lines from a malignant peripheral nerve sheath tumour of the cervix of a rat. - Rat Genome Database

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Neoplastic and non-neoplastic cell lines from a malignant peripheral nerve sheath tumour of the cervix of a rat.

Authors: Yamate, J  Sakamori, M  Kuwamura, M  Kotani, T 
Citation: Yamate J, etal., J Comp Pathol. 2007 Jul;137(1):9-21. Epub 2007 May 29.
RGD ID: 2293746
Pubmed: PMID:17537454   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jcpa.2007.03.004   (Journal Full-text)

A homotransplantable tumour (LY) and cell lines (LY-PPB6 and LY-H12) were established from a spontaneous malignant peripheral nerve sheath tumour (PNST) of the uterine cervix of an F344 rat. Primary and LY tumours consisted of oval or spindle-shaped cells arranged in a flatfield or streaming fashion, and indistinct nuclear palisades were seen. Immunohistochemically, neoplastic cells reacted to vimentin, S-100 protein, neuron-specific enolase (NSE), myelin basic protein (MBP), and glial fibrillary acidic protein (GFAP) in varying degrees, indicating neurogenic derivation. LY-PPB6-induced tumours in syngeneic rats developed cellular whorling patterns reacting particularly strongly to S-100 protein, NSE, MBP and GFAP. Nerve growth factor (NGF) mRNA expression was shown in LY-PPB6 cells by the reverse transcription-polymerase chain reaction (RT-PCR). By contrast, LY-H12 had a normal chromosomal number of 42, and did not produce tumours when injected into syngeneic rats. LY-H12 cells reacted to vimentin and alpha-smooth muscle actin (alpha-SMA), and the alpha-SMA-positive cell number was increased dose-dependently by the addition of transforming growth factor (TGF)-beta1, indicating a myofibroblastic nature. LY-PPB6 cells were neoplastic with properties of PNS cells, whereas LY-H12 cells were non-neoplastic stromal cells showing myofibroblastic differentiation. As TGF-beta1 mRNA expression was shown in both LY-PPB6 and LY-H12 cells by the RT-PCR, the myofibroblastic phenotype of LY-H12 cells may be mediated by paracrine or autocrine signalling in tumour tissues. LY-PPB6 and LY-H12 may prove useful for studies on the pathobiological nature of neoplastic cells and interactions between neoplastic and stromal cells in PNSTs.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ENO2HumanNerve Tissue Neoplasms  ISOEno2 (Rattus norvegicus) RGD 
Eno2RatNerve Tissue Neoplasms  IEP  RGD 
Eno2MouseNerve Tissue Neoplasms  ISOEno2 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Eno2  (enolase 2)

Genes (Mus musculus)
Eno2  (enolase 2, gamma neuronal)

Genes (Homo sapiens)
ENO2  (enolase 2)


Additional Information