RGD Reference Report - Collagenase-3 (MMP-13) is expressed by tumor cells in invasive vulvar squamous cell carcinomas. - Rat Genome Database

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Collagenase-3 (MMP-13) is expressed by tumor cells in invasive vulvar squamous cell carcinomas.

Authors: Johansson, N  Vaalamo, M  Grenman, S  Hietanen, S  Klemi, P  Saarialho-Kere, U  Kahari, VM 
Citation: Johansson N, etal., Am J Pathol. 1999 Feb;154(2):469-80.
RGD ID: 2293612
Pubmed: (View Article at PubMed) PMID:10027405
DOI: Full-text: DOI:10.1016/S0002-9440(10)65293-5

Collagenase-3 (MMP-13) is a human matrix metalloproteinase specifically expressed by invading tumor cells in squamous cell carcinomas (SCCs) of the head and neck. Here, we have further elucidated the role of MMP-13 in tumor invasion by examining its expression in invasive malignant tumors of the female genital tract. Using in situ hybridization, expression of MMP-13 mRNA was detected in 9 of 12 vulvar SCCs, primarily in tumor cells, but not in intact vulvar epithelium, in cervical SCCs (n = 12), or in endometrial (n = 11) or ovarian adenocarcinomas (n = 8). MMP-13 expression was especially abundant in vulvar carcinomas showing metastasis to lymph nodes and was associated with expression of membrane type 1 MMP by tumor cells and gelatinase-A (MMP-2) by stromal cells, as detected by immunohistochemistry. MMP-13 mRNAs were detected in 9 of 11 cell lines established from vulvar carcinomas and in 4 of 6 cell lines from cervical carcinomas, whereas endometrial (n = 10) and ovarian (n = 9) carcinoma cell lines were negative for MMP-13 mRNA. No correlation was detected between MMP-13 expression and p53 gene mutations in vulvar SCC cell lines. However, MMP-13 expression was detected in 5 of 6 vulvar and cervical SCC cell lines harboring HPV 16 or 68 DNA. These results show that MMP-13 is specifically expressed by malignantly transformed squamous epithelial cells, including vulvar SCC cells, and appears to serve as a marker for their invasive capacity.

Annotation

Disease Annotations    
vulva cancer  (IEP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Mmp13  (matrix metallopeptidase 13)

Genes (Mus musculus)
Mmp13  (matrix metallopeptidase 13)

Genes (Homo sapiens)
MMP13  (matrix metallopeptidase 13)


Additional Information