RGD Reference Report - Combined estrogen and ghrelin administration decreases expression of p27(kip1) and proportion of isomyosin type I in the striated urethral and anal sphincters and levator ani of old ovariectomized rats. - Rat Genome Database

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Combined estrogen and ghrelin administration decreases expression of p27(kip1) and proportion of isomyosin type I in the striated urethral and anal sphincters and levator ani of old ovariectomized rats.

Authors: Rizk, DE  Hassan, HA  Al-Marzouqi, AH  Shafiullah, M  Fahim, MA 
Citation: Rizk DE, etal., Int Urogynecol J Pelvic Floor Dysfunct. 2008 May 22;.
RGD ID: 2293611
Pubmed: PMID:18496635   (View Abstract at PubMed)
DOI: DOI:10.1007/s00192-008-0638-3   (Journal Full-text)

We compared estrogen and/or ghrelin effects on pelvic floor muscles in old versus young adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n = 24 x 2) received 42 daily intraperitoneal 17-beta estradiol (10 mug kg(-1)), ghrelin (2 mug kg(-1)), both, or vehicle (n = 6 x 4/group). Cytoplasmic p27(kip1) expression and isomyosin I proportion in striated urethral and anal sphincters and levator ani were measured, respectively, by Western blot analysis and gel electrophoresis with immunohistochemistry of muscle ghrelin receptors and radioimmunoassay of circulating growth hormone. In young adult rats, estrogen significantly decreased cytoplasmic p27(kip1) and isomyosin I signal intensities. In old rats, ghrelin and estrogen/ghrelin significantly decreased both intensities with greater estrogen/ghrelin effect. Ghrelin receptors were not immunostained in any muscle. Estrogen and/or ghrelin significantly increased or decreased, respectively, circulating growth hormone in old and young adult rats. Estrogen/ghrelin administration reversed pelvic floor muscle ageing changes in old ovariectomized rats through growth hormone production.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to estradiol  IEP 2293611 RGD 
response to peptide hormone  IEP 2293611 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cdkn1b  (cyclin-dependent kinase inhibitor 1B)


Additional Information