RGD Reference Report - Glutamate-induced losses of oligodendrocytes and neurons and activation of caspase-3 in the rat spinal cord. - Rat Genome Database

RGD Reference Report - Glutamate-induced losses of oligodendrocytes and neurons and activation of caspase-3 in the rat spinal cord. - Rat Genome Database

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Glutamate-induced losses of oligodendrocytes and neurons and activation of caspase-3 in the rat spinal cord.
Authors:	Xu, GY Liu, S Hughes, MG McAdoo, DJ
Citation:	Xu GY, etal., Neuroscience. 2008 Mar 7;.
RGD ID:	2293318
Pubmed:	PMID:18423997 (View Abstract at PubMed)
PMCID:	PMC2562633 (View Article at PubMed Central)
DOI:	DOI:10.1016/j.neuroscience.2008.02.065 (Journal Full-text)
The toxicity of released glutamate contributes substantially to secondary cell death following spinal cord injury (SCI). In this work, the extent and time courses of glutamate-induced losses of neurons and oligodendrocytes are established. Glutamate was administered into the spinal cords of anesthetized rats at approximately the concentration and duration of its release following SCI. Cells in normal tissue, in tissue exposed to artificial cerebrospinal fluid and in tissue exposed to glutamate were counted on a confocal system in control animals and from 6 h to 28 days after treatment to assess cell losses. Oligodendrocytes were identified by staining with antibody CC-1 and neurons by immunostaining for Neuronal Nuclei (NeuN) or Neurofilament H. The density of oligodendrocytes declined precipitously in the first 6 h after exposure to glutamate, and then relatively little from 24 h to 28 days post-exposure. Similarly, neuron densities first declined rapidly, but at a decreasing rate, from 0 h to 72 h post-glutamate exposure and did not change significantly from 72 h to 28 days thereafter. The nuclei of many cells strongly and specifically stained for activated caspase-3, an indicator of apoptosis, in response to exposure to glutamate. Caspase-3 was localized to the nucleus and may participate in apoptotic cell death. However, persistence of caspase-3 staining for at least a week after exposure to glutamate during little to no loss of oligodendrocytes and neurons demonstrates that elevation of caspase-3 does not necessarily lead to rapid cell death. Beyond about 48 h after exposure to glutamate, locomotor function began to recover while cell numbers stabilized or declined slowly, demonstrating that functional recovery in the experiments presented involves processes other than replacement of oligodendrocytes and/or neurons.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
response to amino acid		IEP		2293318		RGD

Objects Annotated

Genes (Rattus norvegicus)

Casp3 (caspase 3)

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