RGD Reference Report - Immunohistochemistry analysis of platelet-derived growth factor A and B chains and platelet-derived growth factor alpha and beta receptor expression in benign prostatic hyperplasias and Gleason-graded human prostate adenocarcinomas. - Rat Genome Database

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Immunohistochemistry analysis of platelet-derived growth factor A and B chains and platelet-derived growth factor alpha and beta receptor expression in benign prostatic hyperplasias and Gleason-graded human prostate adenocarcinomas.

Authors: Fudge, K  Wang, CY  Stearns, ME 
Citation: Fudge K, etal., Mod Pathol. 1994 Jun;7(5):549-54.
RGD ID: 2292156
Pubmed: PMID:7524068   (View Abstract at PubMed)

The purpose of this study was to determine whether changes in the expression of platelet-derived growth factor (PDGF) and its receptors were associated with prostate cancer. Peroxidase-anti-peroxidase-immunoperoxidase labeling was used to detect the A and B chains and alpha and beta receptors of PDGF in 5 benign prostatic hyperplasias and 13 human prostate adenocarcinomas (Gleason grades 2 to 9). In all 5 benign prostatic hyperplasias, none of the PDGF antibodies (even at high titers, 1:50 dilutions) labeled the epithelial or the stromal cells. In adenocarcinomas, the A chain and alpha receptor antibodies labeled both epithelial and stromal cells. The intensity of the staining was relatively high in low Gleason grade (<6) and low in high Gleason grade (>7) tissue; however, the B chain and beta receptor antibodies failed to label either epithelial or stromal cells in any of the 13 adenocarcinomas. The data suggest that PDGF A and alpha receptor genes may be preferentially turned on in epithelial and stromal prostate tumor cells.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
prostate adenocarcinoma  IEP 2292156; 2292156protein:increased expression:prostateRGD 
prostate adenocarcinoma  ISOPDGFA (Homo sapiens)2292156; 2292156protein:increased expression:prostateRGD 
prostate adenocarcinoma  ISOPDGFRA (Homo sapiens)2292156; 2292156protein:increased expression:prostateRGD 

Objects Annotated

Genes (Rattus norvegicus)
Pdgfa  (platelet derived growth factor subunit A)
Pdgfra  (platelet derived growth factor receptor alpha)

Genes (Mus musculus)
Pdgfa  (platelet derived growth factor, alpha)
Pdgfra  (platelet derived growth factor receptor, alpha polypeptide)

Genes (Homo sapiens)
PDGFA  (platelet derived growth factor subunit A)
PDGFRA  (platelet derived growth factor receptor alpha)


Additional Information