RGD Reference Report - Aberrant platelet-derived growth factor alpha-receptor transcript as a diagnostic marker for early human germ cell tumors of the adult testis. - Rat Genome Database

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Aberrant platelet-derived growth factor alpha-receptor transcript as a diagnostic marker for early human germ cell tumors of the adult testis.

Authors: Mosselman, S  Looijenga, LH  Gillis, AJ  Van Rooijen, MA  Kraft, HJ  Van Zoelen, EJ  Oosterhuis, JW 
Citation: Mosselman S, etal., Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2884-8.
RGD ID: 2292154
Pubmed: PMID:8610136   (View Abstract at PubMed)
PMCID: PMC39728   (View Article at PubMed Central)

Testicular germ cell tumors are the most common form of cancer in young adult males. They result from a derangement of primordial germ cells, and they grow out from a noninvasive carcinoma-in-situ precursor. Since carcinoma in situ can readily be cured by low-dose irradiation, there is a great incentive for non- or minimally invasive methods for detection of carcinoma in situ. We have recently shown that human Tera-2 embryonal carcinoma cells, obtained from a nonseminomatous testicular germ cell tumor, show alternative splicing and alternative promoter use of the platelet-derived growth factor alpha-receptor gene, giving rise to a unique 1.5-kb transcript. In this study we have set up a reverse transcriptase-polymerase chain reaction strategy for characterization of the various transcripts for this receptor. Using this technique, we show that a panel of 18 seminomas and II nonseminomatous testicular germ cell tumors all express the 1.5-kb transcript. In addition, a panel of 27 samples of testis parenchyma with established carcinoma in situ were all found to be positive for the 1.5-kb transcript, while parenchyma lacking carcinoma in situ, placenta, and control semen were all negative. These data show that the 1.5-kb platelet-derived growth factor alpha-receptor transcript can be used as a highly selective marker for detection of early stages of human testicular germ cell tumors.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Germ Cell and Embryonal Neoplasms  IEP 2292154mRNA:splice variant:testisRGD 
Germ Cell and Embryonal Neoplasms  ISOPDGFRA (Homo sapiens)2292154; 2292154mRNA:splice variant:testisRGD 
in situ carcinoma  IEP 2292154mRNA:splice variant:testisRGD 
in situ carcinoma  ISOPDGFRA (Homo sapiens)2292154; 2292154mRNA:splice variant:testisRGD 
seminoma  IEP 2292154mRNA:splice variant:testisRGD 
seminoma  ISOPDGFRA (Homo sapiens)2292154; 2292154mRNA:splice variant:testisRGD 

Objects Annotated

Genes (Rattus norvegicus)
Pdgfra  (platelet derived growth factor receptor alpha)

Genes (Mus musculus)
Pdgfra  (platelet derived growth factor receptor, alpha polypeptide)

Genes (Homo sapiens)
PDGFRA  (platelet derived growth factor receptor alpha)


Additional Information