RGD Reference Report - Suppression of NFkappaB and its Regulated Gene Products by Oral Administration of Green Tea Polyphenols in an Autochthonous Mouse Prostate Cancer Model. - Rat Genome Database

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Suppression of NFkappaB and its Regulated Gene Products by Oral Administration of Green Tea Polyphenols in an Autochthonous Mouse Prostate Cancer Model.

Authors: Siddiqui, IA  Shukla, Y  Adhami, VM  Sarfaraz, S  Asim, M  Hafeez, BB  Mukhtar, H 
Citation: Siddiqui IA, etal., Pharm Res. 2008 Mar 4;.
RGD ID: 2291908
Pubmed: PMID:18317887   (View Abstract at PubMed)
PMCID: PMC3064432   (View Article at PubMed Central)
DOI: DOI:10.1007/s11095-008-9553-z   (Journal Full-text)

PURPOSE: This study examines the role of cell survival/apoptosis related proteins involved in NFkappaB signaling pathways and its associated events in GTP-induced chemoprevention of prostate cancer in TRAMP mice. METHODS: Mice were given 0.1% GTP as drinking fluid. Western blot and immunohistochemical analysis performed to examine NFkappaB and its regulated pathway in response to GTP. RESULTS: Our data demonstrated increased expression of NFkappaB, IKKalpha, IKKbeta, RANK, NIK and STAT-3 in dorso-lateral prostate of TRAMP mice as a function of age and tumor growth and continuous GTP infusion for 32 weeks resulted in substantial reduction in these proteins. The levels of transcription factor osteopontin, a non-collagenous extracellular matrix protein, were also downregulated. Inhibition of NFkappaB signaling is known to activate apoptotic and inhibit anti-apoptotic proteins. Therefore, we analyzed Bax and Bcl(2) levels in the dorsolateral prostate of TRAMP mice fed GTP and observed a shift in balance between Bax and Bcl(2) favoring apoptosis. CONCLUSIONS: Based on the data we suggest that oral consumption of GTP might inhibit osteopontin and NFkappaB signaling that may contribute to induction of apoptosis observed in GTP fed TRAMP mice.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Prostatic Neoplasms  ISOBax (Mus musculus)2291908; 2291908 RGD 
Prostatic Neoplasms  ISOBcl2 (Mus musculus)2291908; 2291908 RGD 
Prostatic Neoplasms  IEP 2291908; 2291908 RGD 
Prostatic Neoplasms  ISOChuk (Mus musculus)2291908; 2291908protein:increased expression:prostate glandRGD 
Prostatic Neoplasms  IEP 2291908; 2291908; 2291908protein:increased expression:prostate glandRGD 
Prostatic Neoplasms  ISOStat3 (Mus musculus)2291908; 2291908protein:increased expression:prostate glandRGD 
Prostatic Neoplasms  ISOTnfrsf11a (Mus musculus)2291908; 2291908protein:increased expression:prostate glandRGD 

Objects Annotated

Genes (Rattus norvegicus)
Bax  (BCL2 associated X, apoptosis regulator)
Bcl2  (BCL2, apoptosis regulator)
Chuk  (component of inhibitor of nuclear factor kappa B kinase complex)
Stat3  (signal transducer and activator of transcription 3)
Tnfrsf11a  (TNF receptor superfamily member 11A)

Genes (Mus musculus)
Bax  (BCL2-associated X protein)
Bcl2  (B cell leukemia/lymphoma 2)
Chuk  (conserved helix-loop-helix ubiquitous kinase)
Stat3  (signal transducer and activator of transcription 3)
Tnfrsf11a  (tumor necrosis factor receptor superfamily, member 11a, NFKB activator)

Genes (Homo sapiens)
BAX  (BCL2 associated X, apoptosis regulator)
BCL2  (BCL2 apoptosis regulator)
CHUK  (component of inhibitor of nuclear factor kappa B kinase complex)
STAT3  (signal transducer and activator of transcription 3)
TNFRSF11A  (TNF receptor superfamily member 11a)


Additional Information