RGD Reference Report - Promoter hypermethylation of multiple genes in hydatidiform mole and choriocarcinoma. - Rat Genome Database

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Promoter hypermethylation of multiple genes in hydatidiform mole and choriocarcinoma.

Authors: Xue, WC  Chan, KY  Feng, HC  Chiu, PM  Ngan, HY  Tsao, SW  Cheung, AN 
Citation: Xue WC, etal., J Mol Diagn. 2004 Nov;6(4):326-34.
RGD ID: 2290418
Pubmed: (View Article at PubMed) PMID:15507671
DOI: Full-text: DOI:10.1016/S1525-1578(10)60528-4

The methylation status of genes in hydatidiform mole and choriocarcinoma and its significance is relatively unexplored. We investigated the methylation status of the promoter regions of six genes, p16, HIC-1, TIMP3, GSTP1, death-associated protein kinase (DAPK), and E-cadherin in 54 hydatidiform moles, five choriocarcinomas, and 10 first trimester placenta by methylation-specific polymerase chain reaction (PCR). Immunohistochemical expression of p16, TIMP3, and E-cadherin, and quantitative real-time RT-PCR of p16 was also performed. Among the six genes examined, the promoter region of four genes (E-cadherin, HIC-1, p16, TIMP3) in choriocarcinoma and three genes (E-cadherin, HIC-1, p16) in hydatidiform mole exhibited aberrant methylation whereas none was hypermethylated in normal placenta. There was a significant correlation between methylation and reduced expression of p16, E-cadherin, and TIMP3 (P < 0.001). Fifteen of the 54 patients with hydatidiform mole developed gestational trophoblastic neoplasia requiring chemotherapy. Promoter hypermethylation of p16 alone, or combined with E-cadherin, was significantly correlated to such development (P = 0.001, 0.0005, respectively). Hypermethylation of multiple genes, especially p16, might be related to the subsequent development of gestational trophoblastic neoplasia.

Annotation

Disease Annotations    
choriocarcinoma  (IEP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Timp3  (TIMP metallopeptidase inhibitor 3)

Genes (Mus musculus)
Timp3  (tissue inhibitor of metalloproteinase 3)

Genes (Homo sapiens)
TIMP3  (TIMP metallopeptidase inhibitor 3)


Additional Information